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Method for extracting flammulina velutipes polysaccharides through enzyme method

A technology for the extraction of Flammulina velutipes polysaccharides and enzymatic methods, which is applied in the field of polysaccharide extraction, can solve the problems of long time, high energy consumption, and low efficiency, and achieve the effects of small activity loss, simple product purification, and great application prospects

Inactive Publication Date: 2019-02-12
周万芳
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although the traditional extraction method is a classic method for extracting polysaccharides, it takes a long time, has low efficiency, and consumes a lot of energy; studies have shown that neutral protease can effectively enzymatically hydrolyze proteins combined with polysaccharides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: Remove the root of Flammulina velutipes, clean and cut into pieces, pulverize, irritate, and pass through a 40-60 mesh sieve; add 1:10 deionized water to the sieved Flammulina velutipes dry powder, stir evenly, and the stirring speed is 5000 rpm, stirring The time is 60 minutes. Add papain with a mass ratio of 20:1 to the obtained Flammulina velutipes solution for hydrolysis; add 95% ethanol to the hydrolyzed solution, precipitate, filter, and dry to obtain a finished product.

Embodiment 2

[0020] Embodiment 2: Remove the root of Flammulina velutipes, clean and cut into pieces, pulverize, irritate, and cross a 60-mesh sieve; Add 1:30 deionized water to the dry powder of Flammulina velutipes sieved, stir evenly, the stirring speed is 7000 rpm, and the stirring time is 90min. Add papain with a mass ratio of 30:1 to the obtained Flammulina velutipes solution for hydrolysis; add 95% ethanol to the hydrolyzed solution, precipitate, filter, and dry to obtain a finished product.

Embodiment 3

[0021] Embodiment 3: Remove the root of Flammulina velutipes, clean and cut into pieces, pulverize, irritate, and cross a 50-mesh sieve; Add 1:20 deionized water to the dry powder of Flammulina velutipes sieved, stir evenly, the stirring speed is 6000 rpm, and the stirring time is 80min. Add papain with a mass ratio of 25:1 to the obtained Flammulina velutipes solution for hydrolysis; add 95% ethanol to the hydrolyzed solution, precipitate, filter, and dry to obtain a finished product.

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PUM

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Abstract

The invention discloses a method for extracting flammulina velutipes polysaccharides through an enzyme method. The method comprises the following steps: (1) removing roots of flammulina velutipes; washing and chopping, and crushing and drying; sieving by a sieve with the size of 40 to 60 meshes; (2) adding de-ionized water into sieved flammulina velutipes dry powder and uniformly stirring; (3) adding papain into a flammulina velutipes solution obtained by step (2) and hydrolyzing; (4) adding 95 percent ethanol into the solution hydrolyzed in step (3); precipitating, filtering and drying to obtain a finished product. The method can be used for effectively extracting the flammulina velutipes polysaccharides.

Description

technical field [0001] The invention relates to a method for extracting polysaccharides, in particular to a method for enzymatically extracting polysaccharides from Flammulina velutipes. Background technique [0002] Flammulina velutipes, also known as winter mushrooms, portobello mushrooms, structure bacteria, green bar bacteria, and hair-stalked money bacteria, belong to Basidiomycotina, layer fungi, agaricaceae, Tricholomaceae, and money bacteria. Flammulina velutipes polysaccharides have been widely valued due to their functions of inhibiting tumors, anti-cancer, and enhancing body immunity. How to improve the extraction rate of Flammulina velutipes polysaccharides and reduce costs has become the key to expanding the use of Flammulina velutipes and increasing its application value. [0003] Although the traditional extraction method is a classic method for extracting polysaccharides, it takes a long time, has low efficiency, and consumes a lot of energy. Studies have sho...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 周万芳
Owner 周万芳
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