Method for extracting flammulina velutipes polysaccharides through enzyme method
A technology for the extraction of Flammulina velutipes polysaccharides and enzymatic methods, which is applied in the field of polysaccharide extraction, can solve the problems of long time, high energy consumption, and low efficiency, and achieve the effects of small activity loss, simple product purification, and great application prospects
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Embodiment 1
[0019] Embodiment 1: Remove the root of Flammulina velutipes, clean and cut into pieces, pulverize, irritate, and pass through a 40-60 mesh sieve; add 1:10 deionized water to the sieved Flammulina velutipes dry powder, stir evenly, and the stirring speed is 5000 rpm, stirring The time is 60 minutes. Add papain with a mass ratio of 20:1 to the obtained Flammulina velutipes solution for hydrolysis; add 95% ethanol to the hydrolyzed solution, precipitate, filter, and dry to obtain a finished product.
Embodiment 2
[0020] Embodiment 2: Remove the root of Flammulina velutipes, clean and cut into pieces, pulverize, irritate, and cross a 60-mesh sieve; Add 1:30 deionized water to the dry powder of Flammulina velutipes sieved, stir evenly, the stirring speed is 7000 rpm, and the stirring time is 90min. Add papain with a mass ratio of 30:1 to the obtained Flammulina velutipes solution for hydrolysis; add 95% ethanol to the hydrolyzed solution, precipitate, filter, and dry to obtain a finished product.
Embodiment 3
[0021] Embodiment 3: Remove the root of Flammulina velutipes, clean and cut into pieces, pulverize, irritate, and cross a 50-mesh sieve; Add 1:20 deionized water to the dry powder of Flammulina velutipes sieved, stir evenly, the stirring speed is 6000 rpm, and the stirring time is 80min. Add papain with a mass ratio of 25:1 to the obtained Flammulina velutipes solution for hydrolysis; add 95% ethanol to the hydrolyzed solution, precipitate, filter, and dry to obtain a finished product.
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