Building method for mouse herpes simplex keratitis model and application

A technology of herpes simplex virus and viral keratitis, which is applied in the field of animal models of human diseases, can solve problems affecting the consistency of the model, the area of ​​corneal damage, and the impact of model application, so as to avoid human operation differences, fix the area, and avoid large difference effect

Inactive Publication Date: 2019-02-15
FIRST AFFILIATED HOSPITAL OF KUNMING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The traditional scratch method uses sharp instruments such as blades, sewing needles, and injection needles. The operation is relatively rough, so it is relatively simple and easy to per

Method used

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  • Building method for mouse herpes simplex keratitis model and application
  • Building method for mouse herpes simplex keratitis model and application
  • Building method for mouse herpes simplex keratitis model and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1. Construction of mouse herpes simplex virus keratitis model

[0034] A method for establishing a mouse herpes simplex virus keratitis model, comprising the following steps:

[0035] 1) Preparation of experimental animals: 6-8 weeks old female Balb / c mice, and check their eyes before operation to ensure that there is no corneal disease;

[0036] 2) Anesthesia of the animal: Inject ketamine into the hind leg muscle, and the mouse is anesthetized after 3-5 minutes.

[0037] Carry out ocular surface anesthesia with oxybucaine hydrochloride eye drop again; The consumption of described ketamine is

[0038] 50mg / kg~100mg / kg,

[0039] 3) Use a corneal trephine with a diameter of 1mm to print a circle in the center of the mouse cornea, scrape off the corneal epithelium in the circle with an iris restorer, and inoculate 2.8*10 3 PFU HSV-1 17 + Viruses, the left eye was inoculated with an equal volume of DMEM medium, and a mouse model of herpes simplex virus keratiti...

Embodiment 2

[0040] Embodiment 2 is different from embodiment 1 in that HSV-1 17+ virus is changed to 2.8*10 3 PFUHSV-1 Mckrae virus.

Embodiment 3

[0041] Embodiment 3 Different from Example 1, the dose of HSV-1 17+ virus is 1.4*10 4 PFU.

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Abstract

The invention discloses a building method for a mouse herpes simplex keratitis model and application. The method comprises the steps that the corneal epithelium in a circle is scrapped off by using acorneal trephine and an iris repositor, the herpes simplex virus SV-1 is inoculated on one eye of an infected mouse, and the mouse herpes simplex keratitis model is built. The depth and area of the corneal defect prepared by using the trephine method are fixed, the built model is more stable and unified, and large differences brought by the model can be avoided. The operation is precise, the influences caused by man-made operation differences can be avoided, and repetition is easy; the building method is suitable for various research directions and various model animals and easy to popularize;the virus is inoculated on the single eye, so that the association with clinic is closer; the corneal defect is large and the viral load is low, so that the death rate is easily controlled within anappropriate range; the animal model is high in repeatability and stability; the building and assessment method is simple in operation, low in requirements for technologies and devices and high in success rate.

Description

technical field [0001] The invention relates to a method for constructing a mouse herpes simplex virus keratitis model and its application, and belongs to the technical field of human disease animal models. Background technique [0002] HSV-1 is a virus with a high prevalence rate, with a global serological prevalence rate of 50%-90%, and there is a trend of increasing year by year. Every year, about 3000-5000 cases of corneal blindness are caused by repeated attacks of HSK, which seriously affects the quality of life of patients; about 750-1000 cases of newborns infected with HSV-1 lead to herpes virus encephalitis, which affects the function of the nervous system and causes Intellectual disability in newborns. The research on the pathogenesis of HSV-1 infection is extremely important for the prevention and treatment of diseases, and a stable, unified and effective animal model is crucial for the subsequent research on its pathogenesis. [0003] In recent years, the resea...

Claims

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Application Information

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IPC IPC(8): A61D1/00A61K39/245A61K49/00
CPCA61D1/00A61K39/245A61K49/0008
Inventor 赵壮红周代娇李涵灵张慧
Owner FIRST AFFILIATED HOSPITAL OF KUNMING MEDICAL UNIV
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