Multifunctional fermentation composite microbial agent for rapid temperature rise and odor removal of livestock and poultry excrement compost, preparation method and application of microbial agent

A technology for fermenting compound bacteria and livestock and poultry manure, which is applied in the field of environmental application microorganisms, can solve the problems that composting does not achieve the ideal effect of compound bacteria, the total number of viable bacteria in the product is not high, and the understanding of interaction is insufficient, so as to improve the quality of compost products, Rapid and effective decomposition of cellulose macromolecules, rapid fermentation and heat production

Inactive Publication Date: 2019-02-19
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The common problem at present is the lack of basic theoretical guidance in the process of screening and cultivating complex microorganisms, which leads to the weak functionality of the screened strains; moreover, the lack of understanding of the fermentation process of compound culture and the interaction between various strains, As a result, the total number of live bacteria in the product is not high, so that the compost does not achieve the ideal effect of using compound bacterial agents to promote the process of decomposing

Method used

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  • Multifunctional fermentation composite microbial agent for rapid temperature rise and odor removal of livestock and poultry excrement compost, preparation method and application of microbial agent
  • Multifunctional fermentation composite microbial agent for rapid temperature rise and odor removal of livestock and poultry excrement compost, preparation method and application of microbial agent
  • Multifunctional fermentation composite microbial agent for rapid temperature rise and odor removal of livestock and poultry excrement compost, preparation method and application of microbial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1: Preparation and application of a multifunctional fermentation compound bacterial agent for rapid heating and deodorizing of livestock manure compost

[0043] Preparation of a multifunctional fermentation compound microbial agent for rapid heating and deodorization of livestock manure compost:

[0044] (1) Slant culture activation: inoculate the strains of Bacillus subtilis, Bacillus mojave, Acinetobacter peter, Bacillus cereus and Nitrobacillus harbinensis BM62 respectively on the corresponding slant culture medium, activate and cultivate the strains activation;

[0045] (2) First-level seed culture: inoculate the activated strains of step (1) into the liquid medium and shake culture to obtain the first-level seed liquid;

[0046] (3) Secondary seed culture: the first-level seed liquid obtained in step (2) is respectively inoculated into a fermentation medium, and fermented in a fermentation tank to obtain a second-level seed liquid;

[0047] (4) Strain solid fermenta...

Embodiment 2

[0065] Example 2: Preparation and application of a multifunctional fermentation compound bacteria agent for rapid heating and deodorizing of livestock and poultry manure compost

[0066] The difference from Example 1 is that the mixing ratio during the preparation of the composite inoculum is: Bacillus subtilis, Bacillus mojave, Acinetobacter peter, Bacillus cereus and Nitrobacillus harbinensis BM62 solid inoculum according to 2:2:1 :1:1 mass ratio mixing.

[0067] Composting raw materials and methods, sampling and determination methods are the same as those in Example 1.

[0068] Experimental results

[0069] Changes in temperature during composting

[0070] The inoculant compost also enters the pyrolysis stage on the second day after composting (> 45°C) (see figure 2 ), the high temperature period lasted for 14 days, which was 3 days longer than that in Example 1.

[0071] Cellulose degradation rate, total N, Content and NH 3 Volatile change

[0072] It can be seen from Table 2 that ...

Embodiment 3

[0075] Example 3: Preparation and application of a multifunctional fermentation compound bacterial agent for rapid heating and deodorization of livestock and poultry manure compost

[0076] The difference from Example 1 is that the mixing ratio during the preparation of the composite inoculum is: Bacillus subtilis, Bacillus mojave, Acinetobacter peter, Bacillus cereus and Nitrobacillus harbinensis BM62 solid inoculum according to 1:2:1 : 2:1 mass ratio mixing.

[0077] Composting raw materials and methods, sampling and determination methods are the same as those in Example 1.

[0078] Experimental results

[0079] Changes in temperature during composting

[0080] The inoculant compost also enters the pyrolysis stage on the second day after composting (> 45°C) (see image 3 ), the high temperature period lasted for 14 days, which was 3 days longer than that in Example 1.

[0081] Cellulose degradation rate, total N, Content and NH 3 Volatile change

[0082] Table 3 Cellulose degradation...

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Abstract

The invention discloses a multifunctional fermentation composite microbial agent for rapid temperature rise and odor removal of livestock and poultry excrement compost, a preparation method and an application of the microbial agent. The composite microbial agent comprises solid microbial agents of bacillus subtilis, bacillus mojavae, acinetobacter pittii, IIa, bacillus cereus and Nitrobacillus harbinensis BM62. The composite microbial agent is a multi-microorganism composite bacterial system with complementary functions, and has multiple effects of heat production in rapid fermentation, nitrogen preservation in biological deodorization, decomposition and degradation of compost materials and the like, so as to enable the livestock and poultry excrement compost to rapidly heat up, the unpleasant odor is obviously reduced, the nitrogen loss in compost is reduced, and organic substances such as cellulose and the like are rapidly and effectively decomposed, and thus the decomposition speedof compost materials is accelerated, the quality of compost products is improved, the agent cab be applied to the production of bio-organic fertilizers, the agriculture production cost is reduced, andmeanwhile, the environmental pollution caused by livestock and poultry excrement is solved, so that the agent has good application value and social significance.

Description

Technical field [0001] The invention belongs to the field of environmental application microorganisms, and in particular relates to a multifunctional fermentation composite microbial agent for rapid heating and deodorization of livestock manure compost, and a preparation method and application thereof. Background technique [0002] In recent years, my country’s animal husbandry has developed rapidly, and the resulting large amount of animal manure not only has not been effectively used, but has also caused serious environmental pollution. Although the use of livestock and poultry manure composting is an ideal way to use waste organic matter as a resource, there are still many problems, such as the volatilization of ammonia during the composting process, which causes air pollution and reduces fertilizer efficiency; the slow heating of the pile leads to organic macromolecules such as cellulose Insufficient degradation will extend the compost maturity time and so on. In response to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F15/00C05F17/00C12R1/125C12R1/07C12R1/01C12R1/085
CPCC05F3/00C05F17/00C12N1/20C05F11/00C05F11/08Y02W30/40
Inventor 徐杰
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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