Multifunctional fermentation composite microbial agent for rapid temperature rise and odor removal of livestock and poultry excrement compost, preparation method and application of microbial agent
A technology for fermenting compound bacteria and livestock and poultry manure, which is applied in the field of environmental application microorganisms, can solve the problems that composting does not achieve the ideal effect of compound bacteria, the total number of viable bacteria in the product is not high, and the understanding of interaction is insufficient, so as to improve the quality of compost products, Rapid and effective decomposition of cellulose macromolecules, rapid fermentation and heat production
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Embodiment 1
[0042] Example 1: Preparation and application of a multifunctional fermentation compound bacterial agent for rapid heating and deodorizing of livestock manure compost
[0043] Preparation of a multifunctional fermentation compound microbial agent for rapid heating and deodorization of livestock manure compost:
[0044] (1) Slant culture activation: inoculate the strains of Bacillus subtilis, Bacillus mojave, Acinetobacter peter, Bacillus cereus and Nitrobacillus harbinensis BM62 respectively on the corresponding slant culture medium, activate and cultivate the strains activation;
[0045] (2) First-level seed culture: inoculate the activated strains of step (1) into the liquid medium and shake culture to obtain the first-level seed liquid;
[0046] (3) Secondary seed culture: the first-level seed liquid obtained in step (2) is respectively inoculated into a fermentation medium, and fermented in a fermentation tank to obtain a second-level seed liquid;
[0047] (4) Strain solid fermenta...
Embodiment 2
[0065] Example 2: Preparation and application of a multifunctional fermentation compound bacteria agent for rapid heating and deodorizing of livestock and poultry manure compost
[0066] The difference from Example 1 is that the mixing ratio during the preparation of the composite inoculum is: Bacillus subtilis, Bacillus mojave, Acinetobacter peter, Bacillus cereus and Nitrobacillus harbinensis BM62 solid inoculum according to 2:2:1 :1:1 mass ratio mixing.
[0067] Composting raw materials and methods, sampling and determination methods are the same as those in Example 1.
[0068] Experimental results
[0069] Changes in temperature during composting
[0070] The inoculant compost also enters the pyrolysis stage on the second day after composting (> 45°C) (see figure 2 ), the high temperature period lasted for 14 days, which was 3 days longer than that in Example 1.
[0071] Cellulose degradation rate, total N, Content and NH 3 Volatile change
[0072] It can be seen from Table 2 that ...
Embodiment 3
[0075] Example 3: Preparation and application of a multifunctional fermentation compound bacterial agent for rapid heating and deodorization of livestock and poultry manure compost
[0076] The difference from Example 1 is that the mixing ratio during the preparation of the composite inoculum is: Bacillus subtilis, Bacillus mojave, Acinetobacter peter, Bacillus cereus and Nitrobacillus harbinensis BM62 solid inoculum according to 1:2:1 : 2:1 mass ratio mixing.
[0077] Composting raw materials and methods, sampling and determination methods are the same as those in Example 1.
[0078] Experimental results
[0079] Changes in temperature during composting
[0080] The inoculant compost also enters the pyrolysis stage on the second day after composting (> 45°C) (see image 3 ), the high temperature period lasted for 14 days, which was 3 days longer than that in Example 1.
[0081] Cellulose degradation rate, total N, Content and NH 3 Volatile change
[0082] Table 3 Cellulose degradation...
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