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A kind of pregnant horse serum gonadotropin freeze-dried powder and preparation method thereof

A technology of gonadotropin and pregnant horse serum, which is applied in freeze-dried delivery, disease delivery, powder delivery, etc. It can solve the problems of weakened interaction between hydrophobic groups, decreased activity of PMSG, and reduced stability of PMSG, so as to induce ovulation, Enhance the effect of action and reduce the effect of structural changes

Active Publication Date: 2021-09-21
NINGBO SANSHENG BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the various stages of the entire freeze-drying of PMSG, there are many factors that can cause changes in the structure of PMSG, such as: the impact of low temperature, as the temperature decreases, the hydration of non-polar groups gradually increases, and the hydrophobic groups in PMSG interact with each other. The effect is weakened, thereby reducing the stability of PMSG; another example, the influence of cooling rate, usually the faster the cooling rate of freeze-drying, the smaller the ice crystals generated inside PMSG, and the size of ice crystals determines the structural activity of PMSG during the drying process
The structural changes of PMSG during the freeze-drying process will lead to a decrease in the activity of PMSG, thereby affecting the effect of PMSG in animals

Method used

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  • A kind of pregnant horse serum gonadotropin freeze-dried powder and preparation method thereof
  • A kind of pregnant horse serum gonadotropin freeze-dried powder and preparation method thereof
  • A kind of pregnant horse serum gonadotropin freeze-dried powder and preparation method thereof

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preparation example Construction

[0027] In one embodiment of the present invention, the preparation method of pregnant horse serum gonadotropin freeze-dried powder comprises the following steps:

[0028] (1) Ingredients: Take pregnant horse serum gonadotropin, lecithin, mannitol, glycidyl phosphate disodium salt and dextran according to the composition ratio, dissolve them in water respectively, then combine the solution, adjust the pH to 7.0~7.5, stir at 200-1000rmp for 20-60min, filter.

[0029] Pregnant horse serum gonadotropin is dissolved with 5-10% of the total volume of water for injection, lecithin is added with 10-15% of the total volume of water for injection, stirred at 200-1000rmp for 20-30min to disperse evenly, mannitol, glycidyl phosphate Disodium salt and dextran are respectively directly dissolved with 10-15% of the total volume of water for injection, and then the dissolved solutions are combined into the batching tank, and water for injection is added to 90-95% of the total volume, and the ...

Embodiment 1

[0036] In the present embodiment, in every 1g of PMSG freeze-dried powder, comprise the composition of following quality:

[0037] Pregnant horse serum gonadotropin: 1000IU, lecithin: 70mg, mannitol: 50mg, glycidyl phosphate disodium salt 40mg, appropriate amount of pH regulator.

[0038] PMSG freeze-dried powder is obtained by the following preparation methods:

[0039] Based on 1000g of freeze-dried powder, weigh each component according to the above composition ratio, dissolve pregnant horse serum gonadotropin with 10% of the total volume of water for injection, add lecithin with 15% of the total volume of water for injection, stir at 800rmp for 25min, and disperse evenly , mannitol and glycidyl phosphate disodium salt were directly dissolved with 15% of the total volume of water for injection, and then each solution was combined into the batching tank, and water for injection was added to 95% of the total volume, and the pH was adjusted with sodium hydroxide. to 7.0, if t...

Embodiment 2

[0041] In the present embodiment, in every 1g of PMSG freeze-dried powder, comprise the composition of following quality:

[0042] Pregnant horse serum gonadotropin: 1000IU, lecithin: 70mg, mannitol: 40mg, dextran 40: 10mg, glycidyl phosphate disodium salt 40mg, appropriate amount of pH regulator.

[0043] PMSG freeze-dried powder is obtained by the following preparation methods:

[0044] Based on 1000g of freeze-dried powder, weigh each component according to the above composition ratio, dissolve pregnant horse serum gonadotropin with 10% of the total volume of water for injection, add lecithin with 15% of the total volume of water for injection, stir at 800rmp for 25min, and disperse evenly , mannitol and glycidyl phosphate disodium salt were directly dissolved with 15% of the total volume of water for injection, and then each solution was combined into the batching tank, and water for injection was added to 95% of the total volume, and the pH was adjusted with sodium hydrox...

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Abstract

The invention belongs to the field of animal husbandry and veterinary technology research, and relates to a pregnant horse serum gonadotropin freeze-dried powder and a preparation method thereof. Each 1g of PMSG freeze-dried powder includes the following components: serum gonadotropin of pregnant horse: 500~2000IU, lecithin: 30~100mg, excipient: 30~80mg, glycidyl phosphate disodium salt 10~ 50mg, appropriate amount of pH regulator.

Description

technical field [0001] The invention belongs to the field of animal husbandry and veterinary technology research, and relates to a pregnant horse serum gonadotropin freeze-dried powder and a preparation method thereof. Background technique [0002] Pregnant Mare Serum Gonadotropin PMSG is a gonadotropin isolated and purified from pregnant mare serum, which is secreted by the trophoblast of the endometrial cup-shaped structure of the early pregnancy mare. PMSG is the only unique gonadotropin among glycoprotein hormones. It has the activities of two gonadotropins, luteinizing hormone and follicle-stimulating hormone, in the same molecule. It is widely used in animal husbandry production: to promote the growth of ovarian follicles; to promote follicular granulosa cells The hyperplasia and estrogen synthesis and secretion stimulate the production of LH receptors on follicles; in female animals, promote the maturation and ovulation of follicles; stimulate the endometrial cells of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/09A61K9/19A61K47/24A61P15/08
CPCA61K9/0019A61K9/19A61K38/09A61K47/24A61P15/08
Inventor 舒佳妮俞通泰王爱珂崔贞亮包汝泼
Owner NINGBO SANSHENG BIOLOGICAL TECH CO LTD