Select breeding method of Cry1C insect-resistant transgenic two-line sterile rice

Abstract

The invention discloses a select breeding method of Cry1C insect-resistant transgenic two-line sterile rice. The select breeding method comprises the steps that (1) rice carrying photo-thermo sensitive genic male sterile genes serves as a receptor, fertile rice containing Cry1C genes and bar genes serves as a donor, and thus BC1F1 seeds are obtained; (2) a weed killer is sprayed to screen positivesingle plants, and planting is conducted; (3) genome DNA of the single-plant rice is extracted, photo-thermo sensitive fertile genes are detected, and the positive single plants and sterile plant materials of the donor are backcrossed to obtain BC2F1 seeds; (4) the step (2) to the step (3) are repeated, BC3F1 is obtained, and selfing is conducted to obtain BC3F2; (5) the BC3F2 is sowed, and low-temperature treatment is conducted in the young ear differentiation stage; (6) after earing, flower fertility and single-ear fructification are observed, and the single plants without pollen are subjected to ear cutting and then eared; (7) regenerated ears are subjected to low-temperature treatment, and selfing is conducted to obtain BC3F3; (8) the step (5) to the step (7) are repeated on the BC3F3, and thus BC3F4 is obtained; and (9) the new insect-resistant two-line sterile rice is bred. According to the select breeding method of the Cry1C insect-resistant transgenic two-line sterile rice, two-line sterile select breeding is completed in four years, and the working efficiency is improved.

Description

technical field [0001] The invention relates to a method for breeding a sterile line, in particular to a method for breeding a two-line sterile line of Cry1C insect-resistant transgenic rice. Background technique [0002] Utilizing heterosis is an important way to increase crop yield. In order to avoid selfing, breeding male sterile line materials is the most economical and effective way to produce hybrids. The success of hybrids depends largely on the selection of sterile lines. Good sterile lines can make hybrids outstanding in yield and quality. However, rice materials with common photothermosensitive genic male sterile genes have defects such as poor insect resistance. [0003] Utilizing the Bt gene to breed new transgenic insect-resistant rice varieties is an environmentally friendly and efficient method to solve the harm of rice stem borers. The insect-resistant Bt gene Cry1C is a dominant gene, and introducing the Cry1C gene into hybrid rice to resist borer damage c...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for breeding Cry1C insect-resistant transgenic rice two-line sterile line, which solves the time-consuming problem of the prior art, can simplify the process, and make the herbicide-resistant gene, insect-resistant gene and non-fertile The breeding gene can be homozygous and stable, complete the selection of two lines of new sterile lines, meet the standard of providing production seeds, and improve work efficiency