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Method for culturing human limbal stem cells

A technology of limbal stem cells and trophoblasts, which is applied in the field of culturing human limbal stem cells, can solve problems such as cross-contamination, difficulty in removing trophoblasts, and insufficient supply of trophoblasts, and achieve the effect of reducing the probability of poisoning

Inactive Publication Date: 2019-03-05
3D GLOBAL BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, limbal stem cells form colonies as the cells expand, pushing trophoblasts away, which may lead to insufficient supply of trophoblasts
In addition, since the limbal stem cells directly contact the trophoblasts during the culture process, it is difficult to completely remove the trophoblasts during harvesting of the limbal stem cells, resulting in the risk of cross-contamination

Method used

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  • Method for culturing human limbal stem cells
  • Method for culturing human limbal stem cells
  • Method for culturing human limbal stem cells

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Embodiment Construction

[0039] In order to make the advantages, spirit and features of the present invention more easily and clearly understood, specific embodiments will be described and discussed in detail with reference to the accompanying drawings. It should be noted that these specific embodiments are only representative specific embodiments of the present invention, and the specific methods, devices, conditions, materials, etc. exemplified therein are not intended to limit the present invention or the corresponding specific embodiments. In addition, each device in the figure is only used to express its relative position and is not drawn according to its actual scale, so it will be described first.

[0040] Please refer to figure 1 , figure 1 It is a flow chart of the steps of a specific embodiment of the method for cultivating human limbal stem cells of the present invention. The invention provides a method for cultivating human limbal stem cells, the steps comprising: S 1 : pretreatment-tro...

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Abstract

The invention discloses a method for culturing human limbal stem cells. The method includes the steps: pretreating a trophoblast; inoculating the trophoblast into a porous membrane; placing the porousmembrane into a container, dividing the container into a first culturing chamber and a second culturing chamber, and enabling the trophoblast to be positioned in the second culturing chamber; injecting a culture medium into the container; placing the human limbal stem cells into the first culturing chamber. Compared with learning and knowing technologies, according tot the method for culturing the human limbal stem cells, the human limbal stem cells are cultured by the aid of a culturing technique of the porous membrane, and the expansion rate of the human limbal stem cells is superior to that of human limbal stem cells cultured by a traditional culture mode. Besides, the used culture medium does not contain toxic dimethyl sulfoxide components, and the toxic killing probability of the cells is reduced.

Description

technical field [0001] The present invention relates to a method of culturing human limbal stem cells, and in particular, to a method of culturing human limbal stem cells using a porous membrane. Background technique [0002] The normality and stability of the corneal surface is extremely important to keep the cornea transparent and maintain normal physiological functions. It is not only a dam between the cornea and conjunctiva, but also a source of corneal epithelial regeneration. Corneal defects are mainly caused by trauma, bacterial infection, chemical burns and contact lenses. According to statistics, about 13 million people around the world suffer from corneal damage or disease that affects vision, and 15 to 2 million new cases are diagnosed every year. Defects in the cornea can lead to a range of eye diseases and even blindness. [0003] The discovery of Limbal Stem Cells (LSCs) is one of the most important advances in ophthalmology in recent decades. For now, trans...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/074C12N5/071
CPCC12N5/0621C12N5/0623C12N2500/25C12N2500/32C12N2500/62C12N2502/1323
Inventor 欧耿良
Owner 3D GLOBAL BIOTECH
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