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Biological seedling raising substrate containing composite microbial flora and preparation method and application of biological seedling raising substrate

A technology for raising seedling substrate and compound flora, applied in the directions of botanical equipment and methods, planting substrate, application, etc., can solve rare problems, and achieve the effect of promoting development and improving seedling effect.

Inactive Publication Date: 2019-03-08
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, a number of biological seedling substrates containing a single strain of bacteria have been developed, but there are not many biological seedling substrate products containing complex flora with excellent effects, especially the biological seedling substrates containing complex flora developed by using different beneficial fungi have been researched.

Method used

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  • Biological seedling raising substrate containing composite microbial flora and preparation method and application of biological seedling raising substrate
  • Biological seedling raising substrate containing composite microbial flora and preparation method and application of biological seedling raising substrate
  • Biological seedling raising substrate containing composite microbial flora and preparation method and application of biological seedling raising substrate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The strains Trichoderma Guizhou NJAU 4742 and Trichoderma aculeatus 38 were inoculated into PDA liquid medium respectively, and cultured at 28°C and 170 rpm for 3 days; the strain Bacillus megaterium L-69 was inoculated into LB medium, and cultured at 30 Cultivate at 170 rpm for 2 days.

[0031] Combining fermentation of wheat straw and pig manure water to obtain decomposed straw as a fermentation product. The decomposed straw is crushed and mixed with peat at a volume ratio of 1:1 to obtain a mixture of decomposed straw powder and peat. The mixture of decomposed straw powder and peat is then mixed with vermiculite and peat. Perlite is compounded at a volume ratio of 2:1:1 to obtain a common seedling substrate.

[0032] The bacterial solution was added to the common seedling culture substrate according to the inoculum amount (volume / dry weight) of 5%, and the moisture was adjusted at 45%, fully mixed, and placed for 1 day, and the content of the target strain reached 10...

Embodiment 2

[0042] The method is the same as the implementation case 1.

[0043] The experiment time is from January 15, 2018 to February 9, 2018. The experiment location is the greenhouse on the first floor of the College of Resources and Environmental Sciences, Nanjing Agricultural University.

[0044] results and analysis

[0045] The fresh weight of the aboveground part is the first index, and the stem diameter, plant height and fresh weight of the underground part are the second index to evaluate the seedling raising effect of different seedling substrates. The results are as follows figure 2 shown. The results of the tomato seedling raising experiment in the second season showed that the seedling raising effect of the biological matrix containing complex flora was the best, followed by the biological matrix containing a single strain, and the effect of CK was the worst. The effect trend of whole sports seedlings is basically the same as that of the first season.

[0046] Compari...

Embodiment 3

[0050] Cucumber seeds are surface sterilized with 10% sodium hypochlorite solution for 10 minutes, then treated in warm water at 55°C for 15 minutes, then soaked in warm water at 30°C for 8 hours, and then placed in a thermostat at 30°C to accelerate germination, keep the humidity above 80%, and keep dark. until germination.

[0051] Treat the seeds to germinate, choose plump, germinated and uniform seeds to sow in the plug trays (choose 50 hole plug trays) that are equipped with mixed substrates, the setting of the experimental group is the same as that of Example 1, one grain per hole. After the sowing is completed, the plug trays are placed in a well-lit greenhouse, and the water in the substrate is replenished from time to time to create an environment suitable for cucumber growth.

[0052] After the cucumbers grew for 25 days, the matrix of the roots of the seedlings was rinsed with clean water, and six growth indicators were measured, including stem diameter, plant heigh...

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Abstract

The invention provides a biological seedling raising substrate containing composite microbial flora and a preparation method and application of the biological seedling raising substrate. The biological seedling raising substrate containing the composite microbial flora is prepared by mixing single effective strains of trichoderma guizhouense NJAU 4742, trichoderma asperellum 38 and bacillus megaterium L-69 according to the volume ratio of 1:1-3:1-3. Firstly strains of the trichoderma guizhouense NJAU 4742 and trichoderma asperellum 38 are inoculated into a PDA PDA liquid culture medium for cultivation respectively; strains of the bacillus megaterium L-69 are inoculated into an LB culture medium for cultivation; a bacterial liquid is added into a common seedling raising substrate accordingto the inoculation amount (volume / dry weight) of 5%, the water content is adjusted to 45%, thorough mixing is carried out, and placement is performed for 1 day, so that the biological seedling raisingsubstrate containing a single strain is prepared, then uniform mixing is carried out according to the volume ratio of 1:1:1, and placement is performed for 1 day, so that the biological seedling raising substrate containing the composite microbial flora is prepared. The biological seedling raising substrate containing the composite microbial flora can significantly promote the seedling raising effect of tomatoes and cucumbers.

Description

technical field [0001] The invention belongs to the high-tech field of agriculture, and relates to a biological seedling-raising matrix containing compound flora, a preparation method and application thereof. Background technique [0002] Composite microorganisms are communities composed of two or more microorganisms. Composite microbial agents have more advantages than single microbial agents. They can adapt to complex environments and have stronger and more comprehensive efficacy. The use of compound bacteria in agricultural production can effectively improve the disease resistance and disease resistance of crops, thereby effectively achieving the effect of weight loss and drug reduction, and can also promote the growth of crops and improve the yield and quality of crops. [0003] With the development of facility agriculture at home and abroad, more and more attention has been paid to industrialized seedling cultivation. At present, domestic seedling substrates are gener...

Claims

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Application Information

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IPC IPC(8): A01G24/20A01G24/22A01G24/28A01G24/15A01G31/00
CPCA01G24/15A01G24/20A01G24/22A01G24/28A01G31/00
Inventor 沈其荣李荣王康张楠沈宗专
Owner NANJING AGRICULTURAL UNIVERSITY
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