A method for preparing methylene blue-containing silica particles with high fluorescence intensity by hydrothermal method

A technology of methylene blue and silicon dioxide, which is applied in the direction of chemical instruments and methods, luminescent materials, etc., to achieve the effect of simple process method, simple device and low price

Inactive Publication Date: 2021-07-16
SHANDONG UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far, the preparation of silica particles containing methylene blue with high fluorescence intensity by hydrothermal method has not been reported.

Method used

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  • A method for preparing methylene blue-containing silica particles with high fluorescence intensity by hydrothermal method

Examples

Experimental program
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Effect test

Embodiment 1

[0013] (1) Add ammonia water and methylene blue in sequence to the mixed solution containing water and ethanol, stir for 15 minutes and then add tetraethyl orthosilicate, where the volumes of water, ethanol, ammonia water and tetraethyl orthosilicate are 17.2:92:2.48: 3.44 milliliters, the concentration of methylene blue is 2.32 millimoles per liter, after stirring for 4 hours, centrifuge to obtain the sediment, the sediment is first washed twice with ethanol, and then washed once with the acetate buffer solution of pH=3.6, wherein, The volumes of ethanol and acetate buffer solution used for washing are 120 milliliters and 20 milliliters respectively, and then the precipitate obtained is dried at 70° C. for 24 hours to obtain silica particles containing methylene blue;

[0014] (2) Put the methylene blue-containing silica particles prepared in step 1 into 35 ml of deionized water so that the concentration of the methylene blue-containing silica particles is 2.86 grams per liter...

Embodiment 2

[0016] (1) Add ammonia water and methylene blue in sequence to the mixed solution containing water and ethanol, stir for 15 minutes and then add tetraethyl orthosilicate, where the volumes of water, ethanol, ammonia water and tetraethyl orthosilicate are 17.2:92:2.48: 3.44 milliliters, the concentration of methylene blue is 2.32 millimoles per liter, after stirring for 4 hours, centrifuge to obtain the sediment, the sediment is first washed twice with ethanol, and then washed once with the acetate buffer solution of pH=3.6, wherein, The volumes of ethanol and acetate buffer solution used for washing are 120 milliliters and 20 milliliters respectively, and then the precipitate obtained is dried at 70° C. for 24 hours to obtain silica particles containing methylene blue;

[0017] (2) Put the methylene blue-containing silica particles prepared in step 1 into 35 ml of deionized water so that the concentration of the methylene blue-containing silica particles is 2.86 grams per liter...

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Abstract

The invention provides a method for preparing silicon dioxide particles containing methylene blue with high fluorescence intensity by using a hydrothermal synthesis method, which belongs to the technical field of chemical raw material production. First add ammonia water, methylene blue and orthosilicate to the mixed solution containing water and ethanol and stir for 4 hours, centrifuge, wash and dry at 70 ° C, and then disperse the prepared silicon dioxide particles containing methylene blue in React in deionized water at 120°C~160°C for 4 hours, centrifuge and wash the obtained sediment and dry at 80°C to obtain silica particles containing methylene blue with high fluorescence intensity. The method has the advantages of simple process, low raw material price and easy industrial production, and the obtained particles have good biocompatibility and high fluorescence intensity.

Description

technical field [0001] The invention discloses a method for preparing silicon dioxide particles containing methylene blue with high fluorescence intensity by using a hydrothermal method, which belongs to the technical field of chemical raw material preparation. Background technique [0002] As one of the important nanomaterials, silica nanoparticles are non-toxic, chemically inert and have good biocompatibility. After proper modification, silica nanoparticles can effectively transfect cells, deliver and control drug molecules in vitro. released, can also label and track biological tissues and organs. Because high fluorescence intensity is crucial for precise tracking of biological targets and in-depth study of biological dynamics, researchers hope that silica-based nanoparticles will have a bright fluorescence (photoluminescence) effect. [0003] However, since silicon dioxide has a wide bandgap (approximately 9eV), it cannot directly make silicon dioxide fluorescent throug...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/06C09K11/00
CPCC09K11/00C09K11/06C09K2211/1037
Inventor 李成峰张伟张广立王文浩陈英铭李金叶王怡恬万冰洁葛筱璐
Owner SHANDONG UNIV OF TECH
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