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Method for determining phenolic acid compounds in vegetable oil

A method of determination and technology of vegetable oil, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of complex processing, large interference, inaccurate quantification, etc., and achieve the effect of fast determination method, accurate quantitative research, and strong practicability

Pending Publication Date: 2019-03-12
CHONGQING UNIV OF EDUCATION
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Problems solved by technology

For mass spectrometry, the high price of mass spectrometry limits the universality of domestic use, while high performance liquid chromatography and capillary electrophoresis electrochemical methods often have long analysis time, low sensitivity, and complicated pretreatment, especially the impurities in the sample. The interference of the target is large, the quantification is not accurate, and it cannot be used for the simultaneous determination of trace phenolic acids in vegetable oils

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  • Method for determining phenolic acid compounds in vegetable oil
  • Method for determining phenolic acid compounds in vegetable oil
  • Method for determining phenolic acid compounds in vegetable oil

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Embodiment Construction

[0026] The present invention will be further explained below in conjunction with specific examples.

[0027] One, under the technical idea of ​​SPE-UHPLC-DAD method, the present invention provides a kind of specific embodiment of the assay method of phenolic acid compound in vegetable oil as follows:

[0028] Sample extraction: Take 5.00g vegetable oil sample in a 50mL polyethylene tube, add 10mL n-hexane solution, vortex for 1.0min, and wait for purification.

[0029] Sample purification: firstly, activate the SPE column with 10.0mL methanol and 10.0mL n-hexane successively, and the flow rate is controlled at 2.0mL / min; after the activation is completed, the sample extract is passed through Diol (diol-based ) solid-phase extraction column, then rinse the extraction column with 10.0mL n-hexane, discard all the effluent, and finally elute with 10.0mL methanol, collect all the eluate, blow it nearly dry with nitrogen, and dissolve the residue in 2.0mL In methanol-water solution...

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Abstract

The invention belongs to the technical field of vegetable oil classification and identification, and particularly discloses a method for determining phenolic acid compounds in vegetable oil. The method for determining nine types of trace phenolic acid compounds in the vegetable oil by the aid of solid phase extraction-high performance liquid chromatography is created on the basis of SEP-UHPLC-DAD(solid phase extraction-ultrahigh performance liquid chromatography-diode array detection) processes. The method has the advantages that conditions such as extraction and purification modes, composition of mobile phase, the flow rate of the mobile phase and the temperatures of chromatographic columns are optimized, accordingly, the method applicable to detecting the vegetable oil with complicatedmatrixes and the phenolic acid compounds is ultimately determined, determined samples are dissolved by n-hexane and are purified by diol-base solid phase extraction columns (Diol-SPE), methyl alcohol-water are used as the mobile phase for the method, gradient elution is carried out by the aid of the method, the flow rate is 0.3 mL / min, the detection wavelengths are 280 nm, and the method is speedyand accurate and is good in repeatability and stability and suitable for detecting the 9 types of phenolic acid compounds in the vegetable oil samples.

Description

technical field [0001] The invention belongs to the technical field of classification and identification of vegetable oils, and in particular relates to a method for determining phenolic acid compounds in vegetable oils. Background technique [0002] Phenolic compounds, including simple phenols, polyphenols and phenolic acids, are important secondary metabolites in plants. At present, there are more than 4,000 kinds of phenolic compounds, which are divided into 12 subcategories. As an important class of phenolic compounds, phenolic acids have attracted much attention due to their antioxidant activity, cancer prevention, anti-inflammation, and cardiovascular disease improvement. Different vegetable oils contain different types and contents of phenolic compounds, which are an important part of the trace components of vegetable oils, which not only contribute to the stability of vegetable oils against oxidation, but also affect their sensory properties and nutritional quality....

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 冉远蕉胡宝丹王锴陈美含曾珍谭博文王强
Owner CHONGQING UNIV OF EDUCATION
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