Method for removing aflatoxin M1 in liquid system by using lactobacillus rhamnosus

A technology of Lactobacillus rhamnosus and aflatoxin, applied to Lactobacillus, bacteria used in food preparation, application and other directions, can solve the problems of degradation method limitation, detoxification effect is not as strong as chemical method, etc., to overcome technical problems , the effect of simple operation

Active Publication Date: 2019-03-15
新疆凯瑞可食品科技有限公司
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  • Application Information

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Problems solved by technology

According to the current research, although the adsorption method can achieve the purpose of removing toxins, the detoxification effect is not as strong as the chemical method and has certain reversibility; the degradation method has good detoxification effect and strong specificity, but its degradation effect is affected by the environment and environmental conditions. Many conditions affect the degradation method

Method used

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  • Method for removing aflatoxin M1 in liquid system by using lactobacillus rhamnosus
  • Method for removing aflatoxin M1 in liquid system by using lactobacillus rhamnosus
  • Method for removing aflatoxin M1 in liquid system by using lactobacillus rhamnosus

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preparation example Construction

[0046] Preparation of test strains:

[0047] Lactobacillus rhamnosus CICC20255

[0048] Lactobacillus rhamnosus CICC20255 was cultured to the end of the logarithm, and the bacterial liquid and 50% (v / v) glycerol were mixed evenly at a volume ratio of 1:1, and stored at -20°C. Before use, Lactobacillus rhamnosus CICC20255 was inoculated in MRS medium at an inoculation amount of 2% (v / v), cultured at 37° C. for 12 hours, and activated by two passages. The activated strains were counted on the plate, and the concentration of the bacterial solution was adjusted to 2.5×10 9 CFU / mL. Centrifuge the bacterial solution in a refrigerated centrifuge at 4°C and 8000r / min for 10min, wash the precipitate twice with phosphate buffer (pH6.0), and place the obtained bacteria in a blast drying oven at 37°C Dry for 1 h and store at -20°C for subsequent use.

[0049] Calculation method of aflatoxin M1 removal rate:

[0050] (1) Detection conditions

[0051] The high-performance liquid chromat...

Embodiment and comparative example 1

[0066] (1) Strain pretreatment

[0067] Get the above-mentioned Lactobacillus rhamnosus CICC20255 test strain prepared above, and do the following treatments respectively:

[0068] High temperature treatment: suspend the bacteria in 4mL pH6.0 PBS, heat at 100°C for 60min;

[0069] Sterilization treatment: suspend the bacteria in 4mL pH6.0 PBS, autoclave at 121°C for 60min;

[0070] Acid treatment: suspend the bacteria in 4mL acid treatment solution and shake for 1h;

[0071] Alkali treatment: Suspend the bacteria in 4mL of alkali treatment solution and shake for 1h.

[0072] The treated suspension was centrifuged at 4° C., 8000 r / min for 10 min, and the precipitate was washed 3 times with PBS (pH 6.0) to obtain bacterial cell precipitate.

[0073] (2) Adjust the pH of the liquid system containing aflatoxin M1

[0074] Configure 25ng / mL AFM1 in PBS and adjust the pH to 6.0.

[0075] (3) Add the Lactobacillus rhamnosus pretreated in step (1) to the liquid system obtained in...

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Abstract

The invention discloses a method for removing aflatoxin M1 in a liquid system by using lactobacillus rhamnosus, and relates to a detoxifying method for toxins. The method comprises the following stepsof (1) pretreating lactobacillus rhamnosus; (2) regulating the pH of the liquid system containing the aflatoxin M1; and (3) adding the pretreated lactobacillus rhamnosus obtained in the step (1) to the liquid system obtained in the step (2), performing shaping cultivation, and separating the lactobacillus rhamnosus from the liquid system, so that the liquid in the aflatoxin M1 is removed. Throughthe method provided by the invention, the aflatoxins M1 in the liquid system can be efficiently removed, and the technical problems existing in the prior art are solved. The method is environment-friendly and simple to operate.

Description

technical field [0001] The invention relates to a toxin detoxification method, in particular to a method for removing aflatoxin M1 in a liquid system by using Lactobacillus rhamnosus. Background technique [0002] Lactobacillus rhamnosus, as one of the most widely studied probiotics in humans, was isolated from healthy human intestines by American scientists in the 1880s. Currently, rhamnose milk is produced and sold in several countries and regions around the world. bacillus products. Lactobacillus rhamnosus is a Gram-positive bacterium belonging to the genus Lactobacillus, which is resistant to digestive environments and can stably attach to the human intestinal tract. Lactobacillus rhamnosus can regulate and improve the intestinal flora, maintain the health of the intestinal microenvironment; promote the growth of bifidobacteria and Lactobacillus acidophilus, and assist their functions; Lactobacillus rhamnosus can relieve human allergies and enhance Body immunity; preve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L5/20
CPCA23L5/28A23V2400/175
Inventor 刘丽波李春韩建春文蓉张国芳吕秋月
Owner 新疆凯瑞可食品科技有限公司
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