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Enterobacterium with high lead tolerance and application of enterobacterium

A technology of Enterobacteriaceae and strains, applied in the field of soil pollution remediation, can solve the problem of less lead-contaminated soil, etc., and achieve the effect of low instrument requirements, great social and economic value, and high adsorption

Inactive Publication Date: 2019-03-19
INST OF AGRI ENVIRONMENT & RESOURCE SHANXI ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, many scholars have made great achievements in the field of microbial remediation, but there are still few reports on the remediation of lead-contaminated soil. The key to microbial remediation must be efficient strains, which is currently the bottleneck of microbial remediation.

Method used

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  • Enterobacterium with high lead tolerance and application of enterobacterium
  • Enterobacterium with high lead tolerance and application of enterobacterium
  • Enterobacterium with high lead tolerance and application of enterobacterium

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Experimental program
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Embodiment 1

[0027] 1) Test material

[0028] (1) The test soil samples were collected from Getaying (sewage treatment plant) in Xiaodian District, Taiyuan City, at 37°43ˊ03"N and 112°34ˊ11"E, on July 6, 2016. The soil samples were collected from the surface layer 0 ~20cm, the soil lead content is high.

[0029] (2) Potato (PDA) medium: 200g of potatoes, 20g of glucose, 15-20g of agar (adding to solid medium), 1000mL of water, peel and chop 200g of potatoes and put them in a pot with deionized water, after boiling Time the time for 30 minutes, filter the boiled potatoes with eight layers of gauze to obtain potato juice, add 20g of glucose, add 15-20g of agar when preparing a solid medium, add water to 1000mL, and sterilize at 121°C for 20 minutes.

[0030] 2) Isolation, purification, domestication and identification of lead-resistant strains

[0031] (1) Weigh 10g of collected fresh soil into a conical flask filled with 90mL of sterile water, 180r min -1 , 30min, shake well to make a so...

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Abstract

The invention discloses an enterobacterium with high lead tolerance and application of the enterobacterium. A strain is the lead-tolerant enterobacterium Enterobacter sp. and is preserved in the ChinaCenter for Type Culture Collection, and a preservation number is CCTCC M 2018095. The enterobacterium and the application have the advantages that the maximum tolerable lead ion concentration of thestrain can reach 2000 mg / L, the strain can normally survive in environments polluted by high-concentration heavy metal, and effects of adsorbing lead ions can be realized by the strain; the enterobacterium is further extremely high in adsorbability for heavy metal lead in water bodies, the adsorption rate can reach 97.82%, and soil and the water bodies which are severely polluted by heavy metal can be remedied by the enterobacterium.

Description

technical field [0001] The invention belongs to the technical field of soil pollution remediation, and relates to a highly lead-resistant Enterobacteriaceae strain and its application. Background technique [0002] As the main industrial base of the country, Shanxi Province is particularly serious about soil heavy metal pollution caused by mining and industrial manufacturing, among which mercury, lead, cadmium, arsenic and hexavalent chromium ion pollution are very serious; secondly, as the country's new energy and The industrialization base, called by a series of reform measures successively issued by the state, has become a resource-based economic transformation comprehensive reform test area, so a large area of ​​polluted sites abandoned by industry and mining quickly appeared. The soil pollution of these polluted sites is extremely serious, so the soil environment Pollution has become a major problem. [0003] At present, bioremediation technology attracts more and more...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/01
CPCB09C1/10C02F3/34C12N1/20C12N1/205C12R2001/01
Inventor 郜春花李建华张敏张强靳东升卢晋晶郜雅静
Owner INST OF AGRI ENVIRONMENT & RESOURCE SHANXI ACAD OF AGRI SCI
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