Method for extracting adipose-derived stem cells

A technology of adipose stem cells and extraction methods, which is applied in the field of adipose stem cell extraction, can solve the problems of large cell damage, affecting the yield of cultured cells, and long digestion time of adipose tissue, achieving the effects of fast passage speed, simple process, and good application prospects
CN109486753AInactive Publication Date: 2019-03-19深圳华云生物科技发展有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
深圳华云生物科技发展有限公司
Publication Date
2019-03-19
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for extracting adipose-derived stem cells. The method includes steps of A, pretreating adipose tissues; B, adding type-I collagenases into the pretreated adipose tissues and carrying out digestion; C, collecting digested adipose cells positioned on lower layers after digestion is carried out for preset time; D, aspirating the type-I collagenases and undigested adipose cells positioned on upper layers and carrying out digestion again; E, repeatedly executing the step C and the step D until pretreated adipose cells are completely digested; F, culturing a plurality of generations of digested adipose cells to obtain passage cells; G, collecting the passage cells and carrying out a plurality of items of cell detection; H, transferring passage culture cells intocell seed banks after the passage cells are qualified in the cell detection items, and storing the passage culture cells in the cell seed banks. The method has the advantages that influence of the digestion time on the adipose-derived stem cells can be effectively lowered in step-by-step digestion modes, and the cell yield or the content of the adipose-derived stem cells can be increased.
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Description

technical field

[0001] The invention relates to the technical field of stem cells, in particular to a method for extracting fat stem cells. Background technique

[0002] Stem cells are the original source of cells for the human body and its various tissues. It has a high potential for self-replication, high proliferation and multilineage differentiation. Stem cell research is cross-penetrating into various fields of modern life science and medicine, and stem cell technology is gradually changing from a laboratory concept to a visible reality. Stem cell research has become a hot spot in life sciences, and it is of great significance to the treatment of human diseases.

[0003] Adipose stem cells are a kind of stem cells with multipotential differentiation potential isolated from adipose tissue in recent years. Adipose stem cells are a sufficient, practical and attractive donor resource for autologous cell replacement, and can be widely used in tissue repair, regeneration, ...

Claims

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