Method for extracting adipose-derived stem cells

A technology of adipose stem cells and extraction methods, which is applied in the field of adipose stem cell extraction, can solve the problems of large cell damage, affecting the yield of cultured cells, and long digestion time of adipose tissue, achieving the effects of fast passage speed, simple process, and good application prospects

Inactive Publication Date: 2019-03-19
深圳华云生物科技发展有限公司
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Problems solved by technology

[0006] In view of the shortcomings of the above-mentioned prior art, the purpose of the present invention is to provide a method for extracting adipose stem cells, which aims to solve the problem in the prior art that the digestion time of adipose tissue is too long, resulting in large cell damage and affecting the later culture and cell yield. question

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  • Method for extracting adipose-derived stem cells
  • Method for extracting adipose-derived stem cells

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Embodiment Construction

[0047] In order to make the object, technical solution and effect of the present invention more clear and definite, the present invention will be further described in detail below with reference to the accompanying drawings and examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0048] The numerical values ​​and their numerical ranges disclosed in the embodiments of the present invention are approximate values ​​rather than definite values. Where error or experimental conditions permit, all values ​​within the error range are included. The numerical ranges provided in the examples of the present invention are intended to represent the relative amounts of the components in the mixture as well as the temperature or other parameter ranges recited in the other process examples.

[0049] The terms "fat", "adipose tissue" and "adipose tissue sample" expressed in the embo...

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Abstract

The invention discloses a method for extracting adipose-derived stem cells. The method includes steps of A, pretreating adipose tissues; B, adding type-I collagenases into the pretreated adipose tissues and carrying out digestion; C, collecting digested adipose cells positioned on lower layers after digestion is carried out for preset time; D, aspirating the type-I collagenases and undigested adipose cells positioned on upper layers and carrying out digestion again; E, repeatedly executing the step C and the step D until pretreated adipose cells are completely digested; F, culturing a plurality of generations of digested adipose cells to obtain passage cells; G, collecting the passage cells and carrying out a plurality of items of cell detection; H, transferring passage culture cells intocell seed banks after the passage cells are qualified in the cell detection items, and storing the passage culture cells in the cell seed banks. The method has the advantages that influence of the digestion time on the adipose-derived stem cells can be effectively lowered in step-by-step digestion modes, and the cell yield or the content of the adipose-derived stem cells can be increased.

Description

technical field [0001] The invention relates to the technical field of stem cells, in particular to a method for extracting fat stem cells. Background technique [0002] Stem cells are the original source of cells for the human body and its various tissues. It has a high potential for self-replication, high proliferation and multilineage differentiation. Stem cell research is cross-penetrating into various fields of modern life science and medicine, and stem cell technology is gradually changing from a laboratory concept to a visible reality. Stem cell research has become a hot spot in life sciences, and it is of great significance to the treatment of human diseases. [0003] Adipose stem cells are a kind of stem cells with multipotential differentiation potential isolated from adipose tissue in recent years. Adipose stem cells are a sufficient, practical and attractive donor resource for autologous cell replacement, and can be widely used in tissue repair, regeneration, ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0667C12N2509/00
Inventor 李伟波杨志华罗世杰梁伟莲
Owner 深圳华云生物科技发展有限公司
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