Bacillus subtilis DDT98806 and application thereof
A technology of DDT98806 and DDT98801, applied in Bacillus subtilis DDT98806 and application fields, can solve problems such as secondary pollution, high cost of chemical treatment, soil disturbance and destruction
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Embodiment 1
[0040] A strain of Bacillus subtilis (Bacillus subtilis) DDT98806 was deposited in the Chinese Center for Type Culture Collection of Wuhan University on October 23, 2018. The preservation number is CCTCCM 2018706, and the preservation address is Wuhan University, Wuhan City, Hubei Province.
[0041] The extraction method of above-mentioned bacillus subtilis DDT98806 is made up of following steps:
[0042] (1) Enrichment
[0043] Weigh the soil sample collected from the ditch of the Pesticide Factory in Jiaxing City, Zhejiang Province, China, and add it to the enriched liquid medium. The mass volume ratio of the soil sample to the enriched liquid medium is 1:10. Shaking in the bed for 48 hours to obtain culture fluid;
[0044] The above-mentioned enrichment liquid medium is to add 15mg DDT in every liter of inorganic salt liquid medium, wherein the inorganic salt liquid medium contains (NH 4 ) 2 SO 4 0.2g, KH 2 PO 4 1.0g, K 2 HPO 4 1.0g, CaCl 2 0.08g, FeSO 4 0.00...
Embodiment 2
[0050] The use of the Bacillus subtilis DDT98806 of Example 1 in the preparation of Bacillus subtilis DDT98806 and Aspergillus terreus DDT98801 composite bacterial agent, the composite bacterial agent is: in 1g of solid composite bacterial agent, the number of colonies contained is 1.5 × 10 9 ~4.5×10 9 CFU Bacillus subtilis DDT98806, the number of colonies is 2.5×10 9 ~5.5×10 9 CFU / g of Aspergillus terreus DDT98801. Aspergillus terreus (Aspergillus terreus) DDT98801, the preservation date is November 22, 2017, the preservation unit is the China Center for Type Culture Collection, the strain preservation number is CCTCC No: M 2017710, and the preservation address is Wuhan University, Wuhan City, Hubei Province. It is disclosed in the Chinese patent whose patent publication number is CN108130281A.
[0051] The number of colonies prepared according to conventional methods was 1.5×10 9 ~4.5×10 9 CFU / g of Bacillus DDT98806 solid bacterial agent; the number of prepared colonies...
Embodiment 3
[0058] Application of Bacillus subtilis DDT98806 and Aspergillus terreus DDT98801 composite bacterial agent in repairing DDT-contaminated soil. Its method of use consists of the following steps:
[0059] (1) Pretreatment of contaminated soil
[0060] Crush the contaminated soil containing DDT, mix it thoroughly, and isolate it from the surrounding uncontaminated soil with a plastic film.
[0061] (2) Remediation of DDT-contaminated soil
[0062] The method for preparing bacillus and Aspergillus terreus solid composite bacterial agent is identical with embodiment 2.
[0063] Insert bacillus and Aspergillus terreus solid composite bacterial agent respectively in the soil after step (1) pretreatment, the inoculum amount of bacillus and Aspergillus terreus composite bacterial agent is 50g / kg, then add urea, K 2 HPO 4 / KH 2 PO 4 Buffer solution and organic fertilizer, so that the mass ratio of C:N:P elements in the soil is 100:10:1, adjust the pH to 4.5-6.5, add water until t...
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