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Normal-temperature mechanical perfusion system capable of extending liver source

A technology of perfusate and normal temperature, applied in the field of medical products, can solve the problems that the oxygenation effect cannot meet the requirements of DCD donor liver preservation, inflammatory mediators induce inflammation or rejection, and long-distance transportation is difficult, so as to prevent ischemia-reperfusion Injury, good market prospects and social welfare value, and the effect of inhibiting inflammatory factor storm

Active Publication Date: 2019-03-26
嘉兴莱普晟医疗科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The polysol perfusion solution invented by Dutch scholars can provide sufficient nutrients for the continuous metabolism of organs; polyethylene glycol was selected as the colloid component, which achieved good results in protecting liver cell function, reducing oxygen consumption, and maintaining microcirculation. It has become a better choice for mechanical perfusion preservation; but its oxygenation effect cannot meet the requirements of DCD donor liver preservation
At present, most studies use whole blood or diluted blood as oxygen carrier perfusion, and the blood cells and plasma components in it have potential negative effects: inflammatory mediators induce inflammation or rejection, intrahepatic microthrombosis, red blood cell disintegration, and risk of infection; Actual operational obstacles such as blood shortage and long-distance transportation difficulties

Method used

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  • Normal-temperature mechanical perfusion system capable of extending liver source
  • Normal-temperature mechanical perfusion system capable of extending liver source
  • Normal-temperature mechanical perfusion system capable of extending liver source

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Composition and Connection Mode of Normal Temperature Mechanical Perfusion System Suitable for Liver Preservation

[0025] Such as figure 1 As shown, in the perfusion system (14), the central processing unit (1) is connected with the peristaltic pump (2), and the output power of the peristaltic pump 2 is controlled by the control unit (20) in the central processing unit (1), so The peristaltic pump (2) controls the perfusion pressure and flow rate of the perfusate through the change of the output power, further, the peristaltic pump (2) is connected with the liver perfusate storage (3), and the liver perfusate storage (3) has two inner and outer layers , a cold and heat regulating tube is arranged between the inner and outer layers, and the cold and heat regulating tube is controlled by the control component (20) to adjust the temperature of the perfusate; a temperature sensor (16) is arranged in the liver perfusate storage (3), Used to monitor the temperatur...

Embodiment 2

[0029] Example 2 Obtaining and Perfusion of Donor Liver

[0030] 1 experimental animal

[0031] 20-25 male Chinese miniature pigs, weighing 30±3Kg. All experimental animals were treated humanely, in compliance with the "Guidelines for the Management and Use of Experimental Animals" promulgated by the National Institutes of Health of the United States, and the experimental protocol was approved by the relevant experimental animal ethics committee.

[0032] 2. Method

[0033] (1) Experimental animals were fasted for 8 hours before operation. Anesthesia was induced by intramuscular injection of sodium pentobarbital, and body weight was weighed and recorded. Take the supine position and fix the limbs on the operating table, inhale high-flow oxygen, prepare the skin, connect the chest lead ECG monitoring, and connect the blood oxygen saturation probe to the end of the pigtail. Scalp needles were used to establish peripheral venous access through the marginal ear veins and fully...

Embodiment 3

[0038] Example 3 Effect of naringenin-7-O-acetate on mechanically perfused liver preserved in vitro

[0039] Naringenin (naringenin) has antibacterial, anti-inflammatory, free radical scavenging, anti-oxidation, cough and expectorant, blood lipid lowering, anti-cancer and anti-tumor, antispasmodic and choleretic, prevention and treatment of liver disease, inhibition of platelet aggregation, anti-atherogenic artery However, whether it can be used to preserve the isolated liver, especially whether its derivative naringenin-7-O-acetate can be used as a component of the perfusion solution, which is beneficial to the mechanical perfusion preservation of the liver, is currently unknown . In order to explore the effect of naringenin-7-O-acetate on mechanical perfusion in vitro preservation of the liver, this embodiment designs two different liver perfusates, the specific components are as described in Table 1:

[0040] Table 1 Composition of perfusate A and perfusate B (1000mL)

[...

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Abstract

The invention provides a normal-temperature mechanical perfusion system capable of extending liver source, wherein each 1000ml of perfusion fluid contains the following components: 200-400mg of naringenin-7-O-acetate, 2-6g of hydroxyl starch, 20-40g of triphosadenine, 15-45g of artificial blood, 2-6g of lecithin, 1-2mg of galactosidase, 1-2mg of acetylgalactosaminase, 30-60mu of insulin, 100-300mlof compound amino acid injection 18AA, 1-5g of antibiotics, 5-30g of glucose, 100-300ml of normal saline, 5-15ml of 10% potassium chloride, 10-30ml of 5% sodium bicarbonate, 10-20ml of 10% calcium chloride, 3-8mg of vitamin B12, 2-6mg of vitamin E, 1-5mg of vitamin C, 1-6mg of dexamethasone, and 5-15mug of alprostadil.

Description

technical field [0001] The invention belongs to the technical field of medical products, and in particular relates to a normal-temperature mechanical perfusate capable of expanding the source of a donated liver. Background technique [0002] China is a country with a high incidence of liver disease. According to the "2013 China Health Statistical Yearbook" and "China Tumor Registration Annual Report", the annual number of new cases of viral hepatitis is 1.37 million, ranking first in Class A and B infectious diseases; the annual number of new cases of liver cancer is 355,000, and the annual death rate The rate of liver disease is as high as 26.26 / 100,000, ranking second in malignant tumors; the annual medical care expenses for liver disease are tens of billions of dollars, which has become a serious problem affecting national health, economic and social development. With the continuous improvement of liver transplantation techniques, perioperative monitoring, and highly effe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 杨子江郑俊李建辉
Owner 嘉兴莱普晟医疗科技有限公司
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