Preparation method of composite bacterial agent for water purification

A compound bacterial agent and water purification technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve problems such as antibiotic residues and food safety, reduce waste of resources, and improve the breeding environment , Excellent degradation effect

Active Publication Date: 2021-04-02
DALIAN NATIONALITIES UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the prevention and control of fish diseases in production practice mainly adopts the method of applying antibiotics or adding antibiotics to bait, which inevitably leads to the problem of antibiotic residues. This is also the main reason for the frequent occurrence of antibiotics exceeding the standard in aquatic products, which seriously affects the food safety

Method used

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  • Preparation method of composite bacterial agent for water purification
  • Preparation method of composite bacterial agent for water purification
  • Preparation method of composite bacterial agent for water purification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Activated strain

[0031] Prepare LB and MRS solid medium, make slant and flat plate, take out the glycerol tubes of the experimental strains Lactobacillus plantarum and Bacillus sime in the refrigerator at -80°C and thaw naturally, mark the slope with a bamboo stick, and culture at 30°C. After 3-7 days of cultivation, they were stored in a 4°C refrigerator for later use.

[0032] (2) Propagation of strains

[0033] Pick the activated experimental strain with a bamboo stick, inoculate it in LB or MRS liquid medium according to the inoculum size of 8%, and cultivate it on a constant temperature shaker at 30°C for 24 hours to obtain the Lactobacillus plantarum strain and Bacillus Simeum ​​liquid;

[0034] (3) Preparation of bacterial suspension

[0035] Pour the liquid of Lactobacillus plantarum and Bacillus sime into a centrifuge tube at a ratio of 2:1, centrifuge at 10000r / min for 10min, mix it evenly with sterile water, prepare a uniformly distributed bacterial s...

Embodiment 2

[0037] The difference between this example and Example 1 is that Lactobacillus plantarum and Bacillus siemum are mixed in a ratio of 1:1 to prepare a composite microbial suspension.

Embodiment 3

[0039] The difference between this example and Example 1 lies in that Lactobacillus plantarum and Bacillus siemum are mixed in a ratio of 1:2 to prepare a composite microbial suspension.

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Abstract

The invention belongs to the field of microbial environmental protection, and particularly relates to a preparation method of a composite microbial agent used for water purification. According to themethod, lactobacillus plantarum and bacillus siamensis are mixed according to a certain proportion to prepare a composite microbial inoculum which has an excellent degradation effect. When the inoculation amount is 8 percent, the degradation rate of ammonia nitrogen salt is 88.57 percent, the degradation rate of nitrite nitrogen is 97.87 percent, the degradation rate of nitrate nitrogen is 92.37 percent, the content of pollution factors such as ammonia nitrogen, the nitrite nitrogen and the nitrate nitrogen in aquaculture can be obviously reduced, the aquaculture environment is effectively improved, and resource waste is reduced.

Description

technical field [0001] The invention belongs to the field of microbial environmental protection, and in particular relates to a preparation method of a composite bacterial agent for water purification. Background technique [0002] With the development of large-scale aquaculture, intensification and intensive farming technology, water quality purification has become a key issue in aquaculture. At present, the prevention and control of fish diseases in production practice mainly adopts the method of applying antibiotics or adding antibiotics to bait, which inevitably leads to the problem of antibiotic residues. This is also the main reason for the frequent occurrence of antibiotics exceeding the standard in aquatic products, which seriously affects the food safety. In addition, how to improve the nutrient utilization rate of bait is also an important issue facing the aquaculture industry. Due to the antagonistic activity and water purification effect of some microorganisms,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/25C12R1/07C02F103/20
CPCC02F3/341C02F2103/20C12N1/20
Inventor 刘秋张美娇于基成袁烽皓
Owner DALIAN NATIONALITIES UNIVERSITY
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