A method for regulating triglyceride content in buffalo mammary gland cells at the molecular level
A technology of triglyceride and molecular level, which is applied in the field of regulating the content of triglyceride in buffalo mammary gland cells at the molecular level, can solve problems such as ambiguity, and achieve the effect of simple method.
Active Publication Date: 2021-11-02
GUANGXI ZHUANG AUTONOMOUS REGION BUFFALO INST
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Problems solved by technology
Exactly how the FADS2 gene plays a role in fatty acid metabolism is not yet clear. Foreign research on FADS family genes is mainly aimed at humans, while domestic research on this gene is mainly focused on chickens. There are few studies on this gene in animals, and it has not yet been studied on buffalo mammary glands. Study on the role of this gene in fatty acid metabolism
Method used
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Embodiment
[0034] 1. Collection of buffalo mammary gland cells:
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The invention relates to the field of biotechnology, in particular to a method for regulating the triglyceride content of buffalo mammary gland cells at the molecular level. The method regulates the FADS2 gene of the buffalo by constructing an overexpression vector and an interference vector of the FADS2 gene. Regulate the content of triglyceride in buffalo mammary gland cells at the scientific level, but at present, there are no related reports at home and abroad that regulate the regulation of the FADS2 gene to regulate the triglyceride content of buffalo mammary gland cells at the molecular level. Make a positive contribution to the regulation and control of the production of low-fat buffalo milk at the molecular level.
Description
【Technical field】 [0001] The invention relates to the technical field of regulating the triglyceride gene of buffalo mammary gland cells, in particular to a method for regulating the content of triglyceride in buffalo mammary gland cells at the molecular level. 【Background technique】 [0002] Buffalo milk is different from the milk produced by well-known dairy cows (commonly black and white cows). It has the advantages of low milk production and high nutritional value. According to the test, the nutritional value of 1 kg of buffalo milk is equivalent to 1.85 kg of black-and-white milk. , vitamin content is very rich, it is a nutritious food suitable for all ages, so it can be called the best in milk. The content of fat, protein and lactose in buffalo milk is several times that of black and white milk, and the content of minerals and vitamins is also dozens of times that of black and white milk and human milk. It is rich in aroma, low in cholesterol, rich in vitamins and tr...
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IPC IPC(8): C12N15/53C12N15/85C12N15/113C12N5/10C12Q1/6851
CPCC12N9/001C12N15/113C12N15/85C12N2310/14C12N2510/00C12Q1/6851C12Q2531/113C12Q2563/107C12Q2545/101
Inventor 马小娅梁贤威邓廷贤庞春英段安琴陆杏蓉梁莎莎
Owner GUANGXI ZHUANG AUTONOMOUS REGION BUFFALO INST