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Method for producing theaflavin by using immobilized polyphenol oxidase

A polyphenol oxidase, theaflavin technology, applied in biochemical equipment and methods, immobilized on/in organic carriers, enzymes and other directions, can solve the adverse effects of theaflavin product quality, polyphenol oxidase utilization Low efficiency, low polyphenol oxidase content, etc., to achieve the effect of promoting and popularizing, good economic and social benefits, and improving utilization rate

Inactive Publication Date: 2019-03-29
JIANGSU DEHE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the low content of polyphenol oxidase in tea leaves, the industrial cost of adopting this method is high, and the utilization efficiency of polyphenol oxidase is low. Not only is it difficult to ensure that the reaction is fully carried out, but also polysaccharides, coffee The quality of theaflavin products will also be adversely affected by other substances

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method utilizing immobilized polyphenol oxidase to produce theaflavins, comprising the following steps:

[0025] a. Take the fresh tea leaves and grind them, add pure water to them one by one, adjust the pH to between 4-6 with a buffer solution, stir at 0°C for 40 minutes to obtain the extract, and carry out coarse filtration and ultra-filtration to the extract Filtration and concentration to obtain crude enzyme liquid;

[0026] b. Immobilizing the above crude enzyme solution by using the activated ion exchange resin to obtain immobilized polyphenol oxidase;

[0027] c. adding ethyl acetate and tea polyphenols to the batching tank successively to obtain a tea polyphenols reaction liquid, wherein the purity of the tea polyphenols is 60%, and the concentration of the tea polyphenols reaction liquid is controlled to be 5%;

[0028] d. the immobilized polyphenol oxidase obtained in step b is placed inside the reaction column, the tea polyphenol reaction solution obtained...

Embodiment 2

[0034] A method utilizing immobilized polyphenol oxidase to produce theaflavins, comprising the following steps:

[0035] a. After taking fresh tea leaves and pulverizing, add pure water to it sequentially, use buffer solution to adjust the pH to between 4-6, stir at 30°C for 10 minutes to obtain the extract, and carry out coarse filtration and ultra-filtration to the extract Filtration and concentration to obtain crude enzyme liquid;

[0036] b. Immobilizing the above crude enzyme solution by using the activated ion exchange resin to obtain immobilized polyphenol oxidase;

[0037] c. adding ethyl acetate and tea polyphenols to the batching tank successively to obtain a tea polyphenols reaction liquid, wherein the purity of the tea polyphenols is 90%, and the concentration of the tea polyphenols reaction liquid is controlled to be 20%;

[0038] d. the immobilized polyphenol oxidase obtained in step b is placed inside the reaction column, the tea polyphenol reaction solution o...

Embodiment 3

[0044] A method utilizing immobilized polyphenol oxidase to produce theaflavins, comprising the following steps:

[0045] a. After fresh tea leaves are taken and pulverized, pure water is sequentially added thereto, the pH is adjusted to between 4-6 by a buffer solution, and the extract is obtained after stirring at 15° C. for 25 minutes, and the extract is subjected to coarse filtration, ultrafiltration Filtration and concentration to obtain crude enzyme liquid;

[0046] b. Immobilizing the above crude enzyme solution by using the activated ion exchange resin to obtain immobilized polyphenol oxidase;

[0047] c. adding ethyl acetate and tea polyphenols to the batching tank successively to obtain a tea polyphenols reaction liquid, wherein the purity of the tea polyphenols is 75%, and the concentration of the tea polyphenols reaction liquid is controlled to be 12%;

[0048] d. the immobilized polyphenol oxidase obtained in step b is placed inside the reaction column, the tea p...

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PUM

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Abstract

The invention discloses a method for producing theaflavin by using immobilized polyphenol oxidase. A carrier is used for adsorbing and immobilizing polyphenol oxidase, a top-down liquid inlet mode isadopted, and a top-down air inlet mode is adopted so as to effectively increase the contact area among air, a tea polyphenol reaction solution and the immobilized enzyme. Meanwhile, the reaction solution is subjected to repeated cycle reaction for a plurality of times so as to fully improve the utilization rate of the polyphenol oxidase and tea polyphenols in the production process of theaflavin,so that the reaction efficiency and the reaction completeness are effectively improved, the yield and the quality of the theaflavin product are improved, the aims of energy conservation and environmental protection are achieved, and the method has good economic and social benefits and is beneficial to popularization and popularization.

Description

technical field [0001] The invention relates to the technical field of theaflavin production, in particular to a method for producing theaflavin by using immobilized polyphenol oxidase. Background technique [0002] Theaflavin is an important quality component and functional component in black tea. It has various biological activities and pharmacological effects such as lowering blood fat, lowering blood pressure, anti-oxidation, anti-radiation, antibacterial, and anti-cancer, so it has good medical and health functions. Theaflavin is the chemical component with the smallest molecular weight, the simplest structure, and the clearest biological activity among the tea pigment series products, and it is the only tea active ingredient with clear pharmacological effects. [0003] Theaflavins are tea polyphenols and oxygen to generate o-quinones under the catalysis of polyphenol oxidase, and o-quinones are further oxidized to biphenol quinone intermediates, and part of the bipheno...

Claims

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Application Information

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IPC IPC(8): C12P17/16C12N11/08
CPCC12N9/0057C12N11/08C12P17/162
Inventor 任雪音刘剑宏浦平南陈仕昌郁步霞秦静凤葛秋霞
Owner JIANGSU DEHE BIOTECH