Method for aqueous phase transfer of hydrophobic material

A hydrophobic, water-phase technology, applied in the direction of mixing methods, chemical instruments and methods, dissolution, etc., can solve problems such as complex synthesis process and low chemical stability

Pending Publication Date: 2019-04-05
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, complex synthetic procedures, low chemical stability, and bioincompatibility are still major issues for the future use of these nanocarriers.

Method used

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  • Method for aqueous phase transfer of hydrophobic material
  • Method for aqueous phase transfer of hydrophobic material
  • Method for aqueous phase transfer of hydrophobic material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] The hydrophobic drug curcumin was dissolved in DMSO to form a 1mmol / L solution, then mixed with 2wt% silk protein nanofibers in a volume ratio of 1:1, and fully mixed on a rotary mixer for 24h;

[0056] Centrifuge the mixed liquid at a high speed of 10000 rcf for 30 minutes to obtain a precipitate, which is silk protein nanofibers loaded with curcumin;

[0057] Add pure water to the obtained precipitate to resuspend, carry out 3 times of centrifugation to clean the residual organic solvent, and finally obtain silk protein nanofibers loaded with hydrophobic drug curcumin, the drug loading rate is 1.4%, the encapsulation rate is 57.0%, and Can be dispersed in water.

Embodiment 2

[0059] The hydrophobic drug curcumin was dissolved in acetonitrile to form a 1mmol / L solution, then mixed with 2wt% silk protein nanofibers in a volume ratio of 1:1, and fully mixed on a rotary mixer for 24h;

[0060] Centrifuge the mixed liquid at a high speed of 10000 rcf for 30 minutes to obtain a precipitate, which is silk protein nanofibers loaded with curcumin;

[0061] Add pure water to the obtained precipitate to resuspend, carry out 3 times of centrifugation to clean the residual organic solvent, and finally obtain silk protein nanofibers loaded with hydrophobic drug curcumin, the drug loading rate is 1.7%, the encapsulation rate is 66.7%, and Can be dispersed in water.

Embodiment 3

[0063] The hydrophobic drug curcumin was dissolved in ethanol to form a 1mmol / L solution, then mixed with 2wt% silk protein nanofibers at a volume ratio of 1:1, and fully mixed on a rotary mixer for 24h;

[0064] Centrifuge the mixed liquid at a high speed of 10000 rcf for 30 minutes to obtain a precipitate, which is silk protein nanofibers loaded with curcumin;

[0065] Add pure water to the obtained precipitate to resuspend, perform 3 times of centrifugation to clean the residual organic solvent, and finally obtain silk protein nanofibers loaded with hydrophobic drug curcumin, the drug loading rate is 2%, the encapsulation rate is 86.6%, and Can be dispersed in water.

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Abstract

The invention provides a method for aqueous phase transfer of hydrophobic materials, including the following steps: A) adding silk fibroin nanofiber solution into the organic solution of hydrophobic materials, thoroughly mixing to obtain a mixed solution, wherein the organic solution of the hydrophobic materials includes hydrophobic materials and organic solvents, the organic solvents are one or more of ethanol, acetonitrile, acetone and dimethyl sulfoxide, B) subjecting mixed solution to high-speed centrifugation to obtain the silk fibroin nanofiber loaded with hydrophobic material, C) resuspending the silk fibroin nanofiber loaded with hydrophobic materials in water and dispersing in aqueous phase. The specific conformation and nanostructured silk fibroin nanofiber carrier of the invention has hydrophilic and hydrophobic blocks, the hydrophobic block captures the hydrophobic material, the hydrphobic material can be effectively loaded on the nanofiber carrier and the solubility of thehydrophobic molecule in the aqueous phase can be increased, therefore, the bioavailability is improved.

Description

technical field [0001] The invention belongs to the technical field of hydrophobic materials, in particular to a method for water-phase transfer of hydrophobic materials. Background technique [0002] Hydrophobic materials (such as drugs, vitamins, etc.) occupy a pivotal position in biomedicine, but their poor solubility leads to a series of problems such as the decrease of drug bioavailability, which greatly hinders their clinical application. Various strategies are currently employed to efficiently convert hydrophobic materials into the aqueous phase. One is to increase the solubility of the material in water through chemical modification, but changes in molecular structure usually destroy activity. Furthermore, some amphiphilic nanocarrier systems have gained more and more interest. These carriers have both hydrophilic and hydrophobic caps, self-assemble into micelles or vesicles in aqueous solution, and then coat hydrophobic molecules to improve water retention. phase ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01F3/12
CPCB01F23/51B01F2101/22
Inventor 吕强张筱旖
Owner SUZHOU UNIV
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