Application of high performance liquid chromatography method to amygdalin detection and characteristic chromatogram

Abstract

The invention relates to the field of amygdalin detection, and particularly relates to an application of a high performance liquid chromatography method to the amygdalin detection and a characteristicchromatogram. The method comprises the following steps of a, preparing a reference solution; b, preparing a test solution; and c, establishing the characteristic chromatogram. The high performance liquid chromatography condition in the step c is a chromatographic column taking octadecyl silane bonded silica gel as a filler; the length of the column is 250 mm; the inner diameter is 4.6 mm; the particle size is 5 microns; the flow speed is 0.6 ml / min; the detection wavelength is 207 nm; and the column temperature is 15 DEG C. The technical problem of quality control of a peach kernel is optimized; a method for establishing the characteristic chromatogram of the peach kernel is provided; according to the polarity of chemical components of the peach kernel, the flowing phase conditions of high performance liquid chromatography are reasonably controlled, and the characteristic chromatogram of the peach kernel is constructed, so that a scientific basis is provided for research of a peach kernel quality system; and other detection conditions of the high performance liquid chromatography are further optimized, so that the method is simple and efficient, and a result is objective, preciseand reliable.

Description

technical field [0001] The invention relates to the field of amygdalin detection, in particular to an application and a characteristic map of high performance liquid chromatography in the detection of amygdalin. Background technique [0002] Peach kernel water decoction is obtained by decocting peach kernel medicinal materials and concentrating them. It has the functions of promoting blood circulation and removing blood stasis, moistening intestines and laxatives, relieving cough and relieving asthma. For amenorrhea dysmenorrhea, abdominal mass in the abdomen, lung abscess and intestinal abscess, injury from falls, dryness of the intestine and constipation, cough and asthma. [0003] As we all know, the efficacy of traditional Chinese medicine does not come from any single active ingredient, but the result of the joint action of various effective part groups. [0004] The quality standards of peach kernel medicinal materials and decoction pieces are included in the 2015 edi...

AI Technical Summary

Problems solved by technology

[0004] The quality standards of peach kernel medicinal materials and decoction pieces are included in the 2015 edition of "Chinese Pharmacopoeia". The identification is limited to shape, microscopic and thin layer identification, and content. There are known components that are interfered by similar structural components during extraction, resulting in difficulty in separation. Detection of defects such as low precision

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