Method for extracting protein from egg white
An extraction method and protein technology, applied in the field of protein extraction, can solve the problems of high protein preparation cost, low production efficiency, toxic reagents, etc., and achieve the effect of increasing economic benefits, improving utilization efficiency, and simple process
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[0028] The preparation method of the present invention includes egg white pretreatment, and then separates six proteins through different extraction steps, and is characterized by the following steps, such as image 3 :
[0029] (1) Take 3000mL of egg white from fresh eggs, mix it with distilled water of equal volume and homogenize it, and mix the egg white evenly at a speed of 3500r / min. Stir at ℃ for 12 hours, then centrifuge to obtain the first precipitate and the first supernatant, first wash off the egg white on the surface of the first precipitate with distilled water, and then use 0.1mol glycine-sodium hydroxide buffer solution with a pH of 9.30 Wash off the surface impurities, and then use 16L of 0.1mol glycine-sodium hydroxide (containing 0.5mol sodium chloride) eluent to elute the lysozyme in the resin and collect it.
[0030] (2) The fresh egg white that does not contain lysozyme is diluted and stirred for 15 minutes with an equal volume of 0.5mol calcium chloride ...
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