A kind of extraction method of protein in egg white
An extraction method and protein technology, applied in the field of protein extraction, can solve the problems of low production efficiency, high protein preparation cost, toxic reagents, etc., and achieve the effects of improving utilization efficiency, increasing economic benefits, and simple process flow
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[0028] The preparation method of the present invention comprises egg white pretreatment, and then separates six proteins through different extraction steps, and is characterized by the following steps, such as image 3 :
[0029] (1) Take 3000mL of egg white of fresh eggs, mix with equal volume of distilled water and homogenize, mix the egg white with a rotating speed of 3500r / min, homogenize for 15min, adjust the pH of the mixed solution to be 9.3, add 1200g of resin, place in 4 Stir at ℃ for 12h, then centrifuge to obtain the first precipitate and the first supernatant, first wash the egg white on the surface of the first precipitate with distilled water, and then use 0.1mol glycine-sodium hydroxide buffer with pH 9.30 Surface impurities were washed away, and then the lysozyme in the resin was eluted with 16 L of 0.1 mol glycine-sodium hydroxide (containing 0.5 mol sodium chloride) eluent and collected.
[0030] (2) dilute the separated fresh egg white without lysozyme with...
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