A kind of extraction method of protein in egg white

An extraction method and protein technology, applied in the field of protein extraction, can solve the problems of low production efficiency, high protein preparation cost, toxic reagents, etc., and achieve the effects of improving utilization efficiency, increasing economic benefits, and simple process flow

Active Publication Date: 2022-03-18
HUAZHONG AGRI UNIV
View PDF11 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] From the above patents and literatures, it can be seen that the chromatographic separation technology adopted by the protein isolate makes its application in industrial production very limited. Adding toxic reagents such as trichloroacetic acid is not only easy to irreversibly denature proteins, but also the safety problems due to the residual toxic reagents are not conducive to its application in the food industry
Second, most researchers need to use different chromatographic devices to improve the purity of various proteins when jointly separating multiple proteins. However, due to the low sample loading and low production efficiency of the chromatographic devices, the cost of protein preparation is too high, which is not conducive to industrialization Production
[0005] In view of the introduction of toxic reagents, low extraction efficiency, and the cost of industrial production in the current process of extracting various proteins from eggs, it is necessary to develop an efficient, green, and economical extraction method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of extraction method of protein in egg white
  • A kind of extraction method of protein in egg white
  • A kind of extraction method of protein in egg white

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0028] The preparation method of the present invention comprises egg white pretreatment, and then separates six proteins through different extraction steps, and is characterized by the following steps, such as image 3 :

[0029] (1) Take 3000mL of egg white of fresh eggs, mix with equal volume of distilled water and homogenize, mix the egg white with a rotating speed of 3500r / min, homogenize for 15min, adjust the pH of the mixed solution to be 9.3, add 1200g of resin, place in 4 Stir at ℃ for 12h, then centrifuge to obtain the first precipitate and the first supernatant, first wash the egg white on the surface of the first precipitate with distilled water, and then use 0.1mol glycine-sodium hydroxide buffer with pH 9.30 Surface impurities were washed away, and then the lysozyme in the resin was eluted with 16 L of 0.1 mol glycine-sodium hydroxide (containing 0.5 mol sodium chloride) eluent and collected.

[0030] (2) dilute the separated fresh egg white without lysozyme with...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for extracting protein in egg white, which mainly adopts the method of salting out precipitation combined with resin adsorption to separate ovomucin, ovotransferrin, ovalbumin, egg inhibitor, and ovomucoid from egg white at one time. and lysozyme, a total of six proteins, the method has a simple process and a short extraction period, not only avoiding the problem of toxic reagent residues, but also greatly improving production efficiency, and is easy to realize industrialization. The invention greatly improves the added value of egg processing and produces significant economic and social benefits.

Description

technical field [0001] The invention relates to the technical field of protein extraction, in particular to a method for extracting protein from egg white. Background technique [0002] my country is a big country for raising poultry, and the output of eggs ranks first in the world. As one of the most common animal foods in human life, eggs are also an important source of nutrition for human beings. In addition, eggs also have a variety of industrial properties, such as foaming, emulsifying and gelling properties, and are widely used in many fields such as medicine, food industry, and daily chemical industry. These characteristics are closely related to various proteins rich in eggs. For example, ovalbumin, which is the most abundant in egg white, accounts for about 54% of the total protein content of egg white, and has good foaming, gelling and emulsifying properties; Ovotransferrin with a content of 12% has a strong ability to chelate iron ions, and has the potential to b...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/36C07K14/79C07K14/77C07K14/465C07K1/30
CPCC12N9/2462C12Y302/01017C07K14/465C07K14/77C07K14/79
Inventor 黄茜纪胜男金永国盛龙马美湖
Owner HUAZHONG AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap