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A method for improving the efficiency of enzymatic modification of wool keratin

A technology of wool keratin and keratinase, which is applied in the field of biochemical modification of textile materials, can solve the problems of low enzymatic modification efficiency of TGase and low lysine content, etc., and achieves improved enzymatic modification efficiency and simple reaction conditions , strong effect with little effect

Active Publication Date: 2020-03-24
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the solid state of wool fiber and the low content of lysine in wool keratin make the efficiency of TGase enzymatic modification of wool not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Amination modification of wool: Weigh the degreased wool, immerse it in an aqueous solution containing 25 mg / L sodium bisulfite at a bath ratio of 1:30, pretreat it in a water bath at 70°C for 1 hour, and then Add propargylamine 60μL / 100mg 羊毛 10 μL of photoinitiator, the initiation power is 90kJ, reacted for 2h, washed with water and dried to obtain aminated wool;

[0025] Determination of mercapto group content in keratin: Shred aminated wool and immerse it in an aqueous solution containing 8M urea and 25mg / L sodium bisulfite, adjust the pH value to 9.5 with 5mol / L sodium hydroxide reagent, and put it in a 90°C oscillating water bath Dissolved in keratin for 6 hours, suction filtered, dialyzed and concentrated to obtain keratin solution. The sulfhydryl content in keratin measured by Ellman's reagent method is 15.3% lower than that of unmodified raw wool keratin;

[0026] (2) TGase enzymatic reaction of aminated wool: immerse wool in 30U / g 羊毛 In 50mM Tris-hydroch...

Embodiment 2

[0029] (1) Amination modification of wool: Weigh degreased wool, immerse it in an aqueous solution containing 25 mg / L mercaptoethanol at a bath ratio of 1:50, pretreat it in a water bath at 70°C for 1 hour, and then add acrylic acid Alkyne amine 60μL / 100mg 羊毛 10 μ L of photoinitiator, the initiation power is 90kJ, reacted for 2 hours, washed and dried with water after taking out to obtain aminated wool; the content of sulfhydryl group in keratin measured by Ellman's reagent method was 14.5% lower than that of unmodified raw wool keratin ;

[0030] (2) TGase enzymatic reaction of aminated wool: immerse wool in 20U / g 羊毛 TGase enzyme in 50mM Tris-hydrochloric acid buffer solution, react at 37°C for 12h, after the reaction, take out the wool, wash it fully, and dry it.

[0031] The concentration of ammonia gas released when TGase catalyzed keratin was measured by indophenol blue method, and the free ammonia content generated by the amination-modified wool was 27.0% higher than t...

Embodiment 3

[0033] (1) Amination modification of wool: Weigh the degreased wool, immerse it in an aqueous solution containing 15mg / L sodium bisulfite, the bath ratio is 1:30, and pretreat it in a water bath at 70°C for 0.5h, Then add propargylamine 40μL / 100mg 羊毛 1. Photoinitiator 20μL, triggering power is 90kJ, reacted for 2h, washed and dried with water after taking out to obtain aminated wool; the content of sulfhydryl group in keratin measured by Ellman's reagent method was 14.8% lower than that of unmodified raw wool keratin ;

[0034] (2) TGase enzymatic reaction of aminated wool: immerse the wool in 50mM Tris-hydrochloric acid buffer solution containing 25U / g wool TGase, and react at 37°C for 12h. After the reaction, take out the wool and wash it thoroughly and dry it.

[0035] The concentration of ammonia gas released when TGase catalyzed keratin was measured by indophenol blue method, and the free ammonia content generated by the amination-modified wool was 27.2% higher than that...

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Abstract

The invention discloses a method for improving the enzymatic modification efficiency of wool keratin, which belongs to the technical field of biochemical modification of textile materials. By utilizing the characteristics of disulfide bonds rich in a structure of the wool keratin, an amino group is introduced into the structure of the keratin by performing a click chemical reaction on a sulfhydrylgroup formed by reduction and propargylamine, so that the number of acting sites of a TGase enzymatic reaction is increased, and the enzymatic modification efficiency of the wool keratin is improved.The method is high in operating reaction efficiency, has no toxicity and is easily controlled, and the problems of residual of by-products, environmental pollution, fabric strength damage and the like caused by a side reaction of the chemical reaction, are avoided, therefore, the method has strong practical value.

Description

technical field [0001] The invention relates to a method for improving the enzymatic modification efficiency of wool keratin, belonging to the technical field of biochemical modification of textile materials. Background technique [0002] As a basic raw material, wool plays an important role not only in the textile industry, but also in the field of recycled materials. As a textile material, wool products have some disadvantages, such as easy felting, difficult care, poor dyeing performance, easy to mildew, etc. And the recycled materials containing wool keratin must also be properly modified before they can be used. Wool and its keratin regeneration materials usually utilize the reactive groups -COOH, -NH contained in wool keratin 2 etc. are modified by chemical methods. However, the chemical reaction process requires the use of a large amount of chemical reagents, and the reaction conditions are harsh, and the damage to the wool fiber is relatively large; in addition, the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): D06M16/00D06M11/54D06M13/325D06M101/12
CPCD06M11/54D06M13/325D06M16/003D06M2101/12
Inventor 崔莉王平袁久刚范雪荣王强孟剑英
Owner JIANGNAN UNIV
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