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A detection kit and cystatin technology, applied in the field of biomedicine, can solve problems such as low sensitivity, and achieve the effects of good stability, improved stability and convenient use
Inactive Publication Date: 2019-04-12
SHANGHAI HIGH TRACK MEDICAL EQUIP CO LTD
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Problems solved by technology
At present, latex particle-enhanced turbidimetry is the most common method, but latex particle-enhanced turbidimetry has problems of low sensitivity, precision, accuracy, linear range, etc., and there are limitations in clinical application.
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Embodiment 1
[0032] A cystatin C detection kit, comprising R1 reagent and R2 reagent, is characterized in that, described R1 reagent is made up of buffer, stabilizer, surfactant, preservative, reaction accelerator, and described R2 reagent is made up of buffer Liquid a, stabilizer a, surfactant a, preservative a, reaction accelerator a.
[0033] The R1 reagent includes the following concentrations of substances: trisaminomethane 3g / L, sodium chloride 9g / L, 1011g / L, 1081g / L, 11755g / L, 1181ml / L;
[0034] The R2 reagent includes the following concentrations of substances: trisaminomethane 3g / L, sodium chloride 9g / L, 1011g / L, 1081g / L, 11755g / L, anticystatin C Serum 1ml / L.
[0035] A preparation method of a cystatin C detection kit, comprising the following steps, taking the configuration of 1L R1 reagent as an example:
[0036] Step 1: Weigh triaminomethane 3g / L, sodium chloride 9g / L, 1011g / L, 1081g / L, 11755g / and 1181ml according to the raw materials of R1 reagent / L;
[0037] Step 2: m...
Embodiment 2
[0043] The components and corresponding contents in the R1 reagent and R2 reagent in a cystatin C detection kit are replaced by:
[0044] The R1 reagent includes the following concentrations of substances: trisaminomethane 5g / L, sodium chloride 9g / L, 1011g / L, 1081g / L, 11755g / L, 1184ml / L;
[0045] The R2 reagent includes the following concentrations of substances: trisaminomethane 3g / L, sodium chloride 9g / L, 1011g / L, 1081g / L, 117 32g / L, anticystatin C Serum 3ml / L.
[0046] The present invention carries out kit performance research through Siemens 1200 type automatic biochemical analyzer,
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Abstract
The invention discloses a Cystatin C detecting kit. The Cystatin C detecting kit comprises an R1 reagent and an R2 reagent, and is characterized in that the R1 reagent is prepared from a buffer solution, a stabilizer, a surfactant, a preservative and a reaction accelerator; and the R2 reagent is prepared from a buffer solution a, a stabilizer a, a surfactant a, a preservative a and a reaction accelerator a. The sensitive and stable Cystatin C detecting kit is convenient to use and easy to operate; and the components participating in a coupled reaction are all very stable, interference of internal and external source substances is avoided, stability is good, and the Cystatin C detecting kit can be used for a long time.
Description
technical field [0001] The invention relates to the technical field of biomedicine, in particular to a cystatin C detection kit. Background technique [0002] Kidney disease is a common clinical disease. So far, early diagnosis, early treatment and reversal of damaged kidney function are the only ways to prevent kidney disease from worsening. The clinical evaluation of the progress and severity of renal disease generally takes renal function as a reference, and glomerular filtration rate (GFR) is the most important indicator reflecting renal function. [0003] Cystatin C is a cysteine protease inhibitor and a low-molecular-weight, basic non-glycosylated protein with a molecular weight of 13.3KD and composed of 122 amino acid residues, which can be produced by all nucleated cells in the body , with a constant production rate. Cystatin C in the circulation is only cleared by glomerular filtration, and is an endogenous marker reflecting changes in glomerular filtration rate...
Claims
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