Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Chip for detecting four thyroid tumor markers and preparation method of chip

A technology for thyroid tumors and markers, which is applied to the chip for detecting the concentration of four thyroid tumor markers and its preparation field, which can solve the problems of high cost, narrow linear range, and low sensitivity of chemiluminescence, and achieve good performance and detection The effect of high rate and simple detection

Inactive Publication Date: 2019-04-12
江苏三联生物工程股份有限公司
View PDF5 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Elisa has the disadvantage of low sensitivity and narrow linear range, and chemiluminescence has the problems of high cost and low throughput

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Chip for detecting four thyroid tumor markers and preparation method of chip
  • Chip for detecting four thyroid tumor markers and preparation method of chip
  • Chip for detecting four thyroid tumor markers and preparation method of chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A chip for detecting common thyroid tumor markers, the chip is prepared through the following steps:

[0034] (1) Black slide pretreatment;

[0035] ① Soak the black slide in the slide pretreatment solution containing NaOH for 16 hours, and then wash it with purified water for 2 to 8 times;

[0036] ② Soak black slides in 1 wt% silane solution (medium: 25% ethanol) for 20 min;

[0037] ③Purge the soaked black glass slides with nitrogen gas, put them in an oven, and bake them at 180°C for 0.2h.

[0038] (2) Spotting capture antibody component solution;

[0039] refer to figure 1 , using automatic machine spotting, dotted into a 4×7 matrix on a black slide; each column is a spot formed by the same capture antibody component solution; the capture antibody component solution includes capture antibodies of calcitonin, Tg, TSH, and CEA component solution.

[0040] (3) blocking the capture antibody component solution;

[0041] The spotted black slide was submerged in blo...

Embodiment 2

[0045] A chip for detecting common thyroid tumor markers, the chip is prepared through the following steps:

[0046] (1) Black slide pretreatment;

[0047] ① Soak the black slide in the slide pretreatment solution containing NaOH for 24 hours, and then wash it with purified water for 2 to 8 times;

[0048] ② Soak the black glass slide in a silane solution with a mass concentration of 0.05% by weight (the medium is 25% ethanol) for 60 minutes;

[0049] ③Purge the soaked black glass slides with nitrogen gas, put them in an oven, and bake them at 140°C for 0.4h.

[0050] (2) Spotting capture antibody component solution;

[0051] refer to figure 1 , using automatic machine spotting, dotted into a 4×7 matrix on a black slide; each column is a spot formed by the same capture antibody component solution; the capture antibody component solution includes capture antibodies of calcitonin, Tg, TSH, and CEA component solution.

[0052] (3) blocking the capture antibody component soluti...

Embodiment 3

[0057] A chip for detecting common thyroid tumor markers, the chip is prepared through the following steps:

[0058] (1) Black slide pretreatment;

[0059] ① Soak the black slide in the slide pretreatment solution containing NaOH for 20 hours, and then wash it with purified water for 2 to 8 times;

[0060] ② Soak the black glass slide in a 0.5wt% silane solution (medium: 25% ethanol) for 30 minutes;

[0061] ③Purge the soaked black glass slides with nitrogen gas, put them into an oven, and bake them at 100°C for 0.6h.

[0062] (2) Spotting capture antibody component solution;

[0063] refer to figure 1 , using automatic machine spotting, dotted into a 4×7 matrix on a black slide; each column is a spot formed by the same capture antibody component solution; the capture antibody component solution includes capture antibodies of calcitonin, Tg, TSH, and CEA component solution.

[0064] (3) blocking the capture antibody component solution;

[0065] The spotted black slide wa...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a protein chip capable of detecting concentration of four common thyroid tumor markers simultaneously. The chip is prepared from the following steps that (1), a black glass sheet is preprocessed; (2), a antibody component solution is captured in a point sampling mode; and (3), the chip is sealed, and therefore the chip is well prepared. The chip is used for measuring the concentration of the thyroid cancer markers in patient blood to determine whether the patient has a thyroid tumor or to determine the recurrence of thyroid tumor after an operation. A kit formed by themethod is convenient to use. Relevance ratio is high. Full-automatic operation of thyroid tumor marker detection can be achieved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a chip for detecting the concentrations of four thyroid tumor markers (Ctn, Tg, TSH and CEA) and a preparation method thereof. Background technique [0002] The thyroid gland is located under the thyroid cartilage in the neck of the human body, on both sides of the trachea, shaped like a butterfly, like a shield, so it is called the thyroid gland. The thyroid is divided into left and right lobes and the isthmus. The left and right lobes are located on the sides of the lower throat and the upper part of the organ. The upper end is from the midpoint of the thyroid cartilage, the lower end reaches the sixth tracheal cartilage ring, and sometimes reaches the suprasternal fossa or retrosternal. [0003] The thyroid has the functions of synthesizing, storing and secreting thyroxine, and its structural unit is follicle. Thyroxine is an organic combination of iodine containing iodine tyro...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/574G01N33/552G01N33/531
CPCG01N33/531G01N33/552G01N33/57484
Inventor 成舜泮锋纲丁俊杰施启尧
Owner 江苏三联生物工程股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com