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Method for cultivating triploid arundo donax

A cultivation method and triploid technology, applied in horticultural methods, botanical equipment and methods, plant gene improvement, etc., can solve the problems of high afforestation cost, high labor intensity, unfavorable industrial production of reed bamboo, etc., and achieve strong resistance reverse effect

Pending Publication Date: 2019-04-16
WUHAN RUNDO BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Existing reed bamboo propagation has methods such as sowing, branching, cuttings, generally based on branching method; Usually in early spring, cut into a clump with 4-5 buds along the four sides of the plant, and then transplant; this method The propagation speed is slow, the seedlings are easy to be poisoned, and the labor intensity is high, the transportation is inconvenient, and the cost of afforestation is high. It is difficult to solve the problem of the shortage of seedlings in a short time, which is not conducive to the industrial production of reed bamboo

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The cultivation method of triploid reed bamboo that the present invention proposes, method step is as follows:

[0022] S1: Parent selection: use Arundos reeds with high stress resistance and fast growth as the source plant parents for polyploid cultivation;

[0023] S2: Cultivation of diploid protocorms: aseptically sow the seeds of the source plants in a solid medium, and cultivate them under light. The conditions for light culture are: the culture temperature is within the range of temperature difference between day and night at 25-18°C, and the light time is 10 hours. / d, light intensity 2000Lux, color temperature 4500k, protocorm finally obtained, the formula of the solid medium is: 1 / 2MS+NAA 0.1mg / L+sucrose 6g / L+coconut milk 5g / L+naphthaleneacetic acid 0.05mg / L+sea foam Stone 0.5g / L + activated carbon 0.8g / L, the pH of the medium is 5;

[0024] S3: Fusion and differentiation of diploid cells: Protoplasts were prepared by enzymatic hydrolysis of the protocorms obt...

Embodiment 2

[0029] S1: Parent selection: use Arundos reeds with high stress resistance and fast growth as the source plant parents for polyploid cultivation;

[0030] S2: Cultivation of diploid protocorms: aseptically sow the seeds of the source plants in a solid medium, and cultivate them under light. The conditions for light culture are: the culture temperature is within the range of temperature difference between day and night at 25-18°C, and the light time is 14 hours. / d, light intensity 3000Lux, color temperature 5500k, protocorms are finally obtained, the formula of the solid medium is: 1 / 2MS+NAA 0.15mg / L+sucrose 8g / L+coconut milk 10g / L+naphthaleneacetic acid 0.1mg / L+sea foam Stone 1.5g / L + activated carbon 2.0g / L, the pH of the medium is 6;

[0031] S3: Fusion and differentiation of diploid cells: Protoplasts were prepared by enzymatic hydrolysis of the protocorms obtained in S2, and cell fusion was performed by cell fusion instrument or polyethylene glycol PEG method to obtain ho...

Embodiment 3

[0036] S1: Parent selection: use Arundos reeds with high stress resistance and fast growth as the source plant parents for polyploid cultivation;

[0037] S2: Cultivation of diploid protocorms: aseptically sow the seeds of the source plants in a solid medium, and cultivate them under light. The conditions for light culture are: the culture temperature is within the range of temperature difference between day and night at 25-18°C, and the light time is 12 hours. / d, light intensity 2500Lux, color temperature 5000k, protocorms are finally obtained, the formula of the solid medium is: 1 / 2MS+NAA 0.12mg / L+sucrose 7g / L+coconut milk 8g / L+naphthaleneacetic acid 0.08mg / L+sea foam Stone 1.0g / L + activated carbon 1.4g / L, the pH of the medium is 5.5;

[0038] S3: Fusion and differentiation of diploid cells: Protoplasts were prepared by enzymatic hydrolysis of the protocorms obtained in S2, and cell fusion was performed by cell fusion instrument or polyethylene glycol PEG method to obtain ...

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Abstract

The invention discloses a method for cultivating triploid arundo donax. The method the steps of S1, using the high-resistance and fast-growing arundo donax as a source plant of polyploid culture; S2,sterilizing and sowing source plant seeds in a solid medium, conducting light culture, and finally obtaining protocorm; S3, preparing protoplast by using protocorm obtained in S2, conducting cell fusion, obtaining homologous double diploid cells, culturing into callus using the medium, inducing the tetraploid callus to differentiate, and obtaining the tetraploid arundo donax seedlings; S4, conventionally hardening the tetraploid seedlings, and cultivating for flowering; S5, when the tetraploid arundo donax is flowered, using the tetraploid arundo donax as a female plant, using a diploid arundodonax as a male plant, conducting sexual hybridization, and after the hybridization, obtaining the seeds which are triploid arundo donax; S6, conducting non-symbiotic germination and cultivation, bulb induction, seedling differentiation and transplanting on the triploid arundo donax seeds. The arundo donax has strong resistance to stress.

Description

technical field [0001] The invention relates to the technical field of reed bamboo cultivation, in particular to a method for cultivating triploid reed bamboo. Background technique [0002] Arundodonax (Arundodonax) is a perennial plant belonging to the Gramineae family. It has well-developed rhizomes, thick, upright, tough stalks, many nodes, and often branches. It can be used in various industries such as feed, ethanol, papermaking, power generation, and environmental restoration. . [0003] Existing reed bamboo propagation has methods such as sowing, branching, cuttings, generally based on branching method; Usually in early spring, cut into a clump with 4-5 buds along the four sides of the plant, and then transplant; this method The propagation speed is slow, the seedlings are easy to be poisoned, and the labor intensity is high, the transportation is inconvenient, and the cost of afforestation is high. It is difficult to solve the problem of shortage of seedlings in a s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01H1/08A01H1/02
CPCA01H1/02A01H1/08A01H4/001A01H4/008
Inventor 戴军钟守俊胡国卿明照
Owner WUHAN RUNDO BIOTECHNOLOGY CO LTD
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