DNA tetrahedron-wogonin compound and preparation method and application thereof
A technology for wogonin and complex, which is applied in the field of DNA tetrahedron-wogonin complex and its preparation, achieves the effects of improving bone damage, inhibiting inflammatory response, and promising industrialization prospects
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0040] Preparation and Characterization of Example 1 DNA Tetrahedron
[0041] 1. Method
[0042] 1.1 Preparation method of DNA tetrahedron (TDN)
[0043] TDN is self-assembled by four uniquely designed DNA single strands (S1, S2, S3, S4) through a fast, simple and specific PCR program (95°C for 10min, rapid cooling to 4°C for 20min, long-term storage at 4°C) Synthetic. The four single strands were added to 96 μl TM buffer (10 mM Tris-HCl, 50 mM MgCl 2 , pH 8.0) in a 200μl EP tube, the reaction solution was heated to 95°C for 10min, and then rapidly cooled to 4°C to synthesize TDN.
[0044] 1.2 The specific sequences of the four DNA single strands are as follows:
[0045]
[0046]
[0047] 1.3 Characterization of TDN by polyacrylamide gel electrophoresis, dynamic light scattering (DLS), atomic force microscopy (AFM), transmission electron microscopy (TEM) and charge determination:
[0048] ①PAGE: The successfully synthesized TDN was firstly characterized by 8% polyac...
Embodiment 2
[0055] Example 2 Preparation and characterization of wogonin (W) solution and TDN-wogonin complex (TWC)
[0056] 1. Preparation of W solution and TWC
[0057] Preparation of W solution: W was purchased from Coolaber (China, Beijing), and was directly dissolved in dimethyl sulfoxide (DMSO) to obtain a wogonin solution.
[0058] Preparation of TWC: Take an appropriate amount of wogonin solution, double distilled water (or TM buffer) and TDN solution, mix, so that the concentration of wogonin in the mixed solution is 10 μM, 25 μM and 50 μM, and the concentration of TDN is 250 nM; then Incubate directly on a constant temperature shaker at 4°C for 8h.
[0059] 2. Characterization of W
[0060] Zeta potential (ZetasizerNano ZS90) was measured for W, and the morphology, structure and size of W were characterized by AFM and TEM. The results show that the potential of W is negative about 17. Combining AFM and TEM results show that W is uniformly distributed tiny particles with a si...
experiment example 1
[0066] Experimental Example 1 Cell Experiment
[0067] 1. Cell type: Chondrocytes.
[0068] 2. Experimental setup: when the chondrocytes were subcultured to the P1 generation, the digested chondrocytes were cultured in a 6-well plate, and after 12 hours of culture with 10% serum, they were cultured with 8%, 2%, and 1% serum in gradients. After dropping to 1%, do the following:
[0069] Control group: cultivated for 38 hours.
[0070] Model group: After cultured for 14 hours, IL-1β (concentration: 10 ng / ml) was added to treat for 24 hours.
[0071] Experimental group (TDN, wogonin and TWC): After 12 hours of culture, TDN, wogonin or TWC solution was added for 2 hours, and then IL-1β (concentration: 10 ng / ml) was added to each group for 24 hours.
[0072] In the experimental examples, "inflammatory chondrocytes" refer to chondrocytes treated with IL-1β (concentration: 10 ng / ml) for 24 hours.
[0073] 3. Relevant detection methods and results
[0074] (1) Detection of TDN en...
PUM
Property | Measurement | Unit |
---|---|---|
Particle size | aaaaa | aaaaa |
Particle size | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com