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Detection method of polydatin and its metabolites in biological samples

A technology for biological samples and metabolites, applied in the field of detection of polydatin and its metabolites

Active Publication Date: 2021-09-14
BEIJING UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is only for the separation and analysis of resveratrol glycosides and resveratrol in plasma, and does not involve the separation of a large number of metabolites of resveratrol glycosides in the body

Method used

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  • Detection method of polydatin and its metabolites in biological samples
  • Detection method of polydatin and its metabolites in biological samples
  • Detection method of polydatin and its metabolites in biological samples

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] 1 Experimental instruments and materials

[0057] DIONEX Ultimate 3000 ultra-high performance liquid chromatography analysis system: American Thermo Fisher Company;

[0058] LTQ-Orbitrap XL mass spectrometer: American Thermo Fisher Company, equipped with electrospray ion source (ESI) and Xcalibur 2.1 workstation;

[0059] Grace Pure TM SPEC 18 -Low SPE cartridge (500mg / 3mL);

[0060] Milli-Q Synthesis ultrapure water purification system: Millipore, USA;

[0061] R200D electronic analytical balance (1 / 100,000): German Sartorius company;

[0062] KQ-250DE CNC ultrasonic cleaner: Kunshan Ultrasonic Instrument Co., Ltd.

[0063] Polydatin reference substance: product of Chengdu Master Biological Technology Co., Ltd. (purity greater than 98%);

[0064] Formic acid (chromatographically pure): Germany Merck company;

[0065] Methanol (mass spec pure) and acetonitrile (mass spec pure): American Thermo Fisher Company.

[0066] Sprague Dawley (SD) rats (male, weighing 22...

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Abstract

The invention discloses a method for detecting polydatin and its metabolites in biological samples, which is characterized in that it comprises the following steps: (1) processing the biological samples; and (2) using ultra-high performance liquid chromatography-mass spectrometry The instrument detects the solution to be tested. The detection method of the invention can detect more polydatin metabolites in biological samples, thereby laying the foundation for elucidating the metabolic pathway of polydatin.

Description

technical field [0001] The invention relates to a detection method for polydatin and its metabolites in biological samples. Background technique [0002] Polygonum cuspidatum is the dry rhizome and root of Polygonum cuspidatum Sieb.et Zucc. Polydatin isolated from Polygonum cuspidatum is considered to be the main natural active ingredient of Polygonum cuspidatum and has strong biological activity. Modern pharmacological studies have shown that polydatin has significant effects on cardiomyocytes, vascular smooth muscle cells, anti-platelet aggregation, and improving microcirculation. In addition, it can also reduce tissue and organ damage caused by various factors. Lipid peroxidation and other effects are often used as quality control indicators for Polygonum cuspidatum or polydatin compound preparations. However, at present, the metabolic process and metabolites of polydatin in vivo are still unclear. Continue to study further. [0003] The document "LC-MS / MS Determinati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 张加余梅晓丹王喻淇
Owner BEIJING UNIV OF CHINESE MEDICINE