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Method for preparing animal erythrocyte preserving fluid and application of animal erythrocyte preserving fluid

A preparation method and red blood cell technology, applied in the field of red blood cell preservation, can solve problems such as red blood cell fragmentation and hemolysis, and achieve the effects of easy-to-obtain reagent materials, simple configuration methods, and economical, energy-saving and resource-saving effects

Inactive Publication Date: 2019-04-19
EAST CHINA NORMAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the sodium ion content in the blood of camelids is much higher than that in human plasma, when the human red blood cell preservation solution is directly used to preserve the red blood cells of camelids, the erythrocytes are broken and hemolyzed due to the osmotic pressure difference, so it is necessary to prepare special and camelid The erythrocyte preservation solution of Camelidae preserves the erythrocytes of camelids, so far there is no public literature report on the erythrocyte preservation solution of camelids

Method used

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  • Method for preparing animal erythrocyte preserving fluid and application of animal erythrocyte preserving fluid
  • Method for preparing animal erythrocyte preserving fluid and application of animal erythrocyte preserving fluid
  • Method for preparing animal erythrocyte preserving fluid and application of animal erythrocyte preserving fluid

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Embodiment 1

[0017] Prepare according to the formula table in Table 1 and the concentration in Table 2 below, weigh each component and dissolve it in 1000ml of deionized water, and filter it with a 0.22μm filter membrane to prepare animal red blood cell preservation solution for use .

[0018] Table 1 Preservation solution formula table

[0019] name

Content (mmol / L)

Sodium citrate

90

glucose

120

citric acid

15.5

Sodium dihydrogen phosphate

18

Mannitol

20

ATP

1

Glutathione

1

[0020] Table 2 Concentration table of each component substance

[0021] name

Embodiment 2

[0023] Carry out the in vitro preservation of camel erythrocytes by the preservation solution prepared in the above-mentioned embodiment 1 according to the following steps:

[0024] a. Centrifuge the collected camel anticoagulated whole blood at 3000rpm for 5min, then remove the plasma with a 5ml pipette, and use a 1ml micropipette to remove the buffy coat as much as possible.

[0025] b. Mix the remaining red blood cells according to the volume ratio of camel red blood cells:maintenance solution is 1:2.

[0026] c. Divide the above camel erythrocyte mixture into 5ml non-anticoagulated blood collection tubes.

[0027] d. Store the above-mentioned subpackaged camel erythrocyte test tubes in a refrigerator at 4°C.

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Abstract

The invention discloses a method for preparing animal erythrocyte preserving fluid and application of the animal erythrocyte preserving fluid. The method is characterized in that the erythrocyte preserving fluid is prepared from ATP, glutathione, sodium citrate, glucose, citric acid, sodium dihydrogen phosphate, mannitol and deionized water according to the molar ratio of 1:1:(80-100):(110-130):(12-20):(15-22):(16-25):(545-565), filtering is carried out through a filtering membrane of 0.22 micrometer, and the animal erythrocyte preserving fluid is prepared; the volume ratio of the preserving fluid serving as a reagent for clinical erythrocyte infusion of camelidae animals to the preserving fluid for in-vitro preservation is 1:(1.5-2.5). Compared with prior art, the in-vitro effective animal erythrocyte preserving time reaches 30 days, the preserving fluid can be used for clinical erythrocyte infusion of the camelidae animals, the preparation method is simple, the reagent materials areeasy to obtain, and the method is an efficient and green preparation method according to energy resource saving.

Description

technical field [0001] The invention relates to the technical field of red blood cell preservation, in particular to a preparation method and application of camelid red blood cell preservation solution. Background technique [0002] As economic animals, camelids have a certain range of breeding scale. Some species of camelids, such as wild camels, are endangered wild animals. Blood transfusion therapy is an important means in the process of first aid for endangered species and veterinary treatment of economic animals, but currently there is no There is no in vitro preservation solution for erythrocytes from camelids. The lifespan of human erythrocytes is about 120 days. The membrane fragility of damaged or aging erythrocytes increases, resulting in the exposure of antigens. The exposed antigens combine with self-IgG and are eventually phagocytized by macrophages. In addition, the aging of red blood cells leads to a decrease in the content of CD47 on the surface, which also ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 赵俸涌朱自严钱旻李勤郭忠慧杨颖叶璐夷张佳敏王晨杨启修
Owner EAST CHINA NORMAL UNIV