A method for culturing isolated embryos with inner testa for measuring viability of sorbifolia sorbifolium seeds
A technology of viability and inner testa, which is applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems of poor repeatability, inability to accurately measure the viability of X. Small damage, improved seed germination rate, low cost effect
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Embodiment 1
[0032] A method for culturing isolated seed coat in vitro embryos of rare woody economic crops Xantho sorbifolium seed viability, the batch of seeds comes from the Xantho sorbifolium base of the Qilian Mountain Water Conservation Forest Research Institute in Zhangye City, Gansu Province, comprising the following steps:
[0033] Step 1, cleanness processing: screen with a 5mm sieve to remove impurities and other plant seeds to obtain the clean seeds of X. sorbifolium;
[0034] Step 2, get test sample: from the net seed of step 1 clarity process, get 500 test samples at random, be divided into 4 repetitions, each repeats 125 seeds;
[0035] Step 3, sample pre-wetting: put the four repeated test samples taken in step 2 into a beaker, and soak in water at 15°C-25°C for 12 hours;
[0036] Step 4, remove the exocarp mechanically: Clamp the pre-wetted seed sample in step 3 with pliers, remove the exocarp mechanically, leave the endocarp and embryo, do not damage the embryo, for dryne...
Embodiment 2
[0042] In the present embodiment, a kind of in vitro embryo culture method with in-band testa of rare woody economic crop sorbifolium sorbifolium seed viability is measured, and the sample is from Inner Mongolia, and step 1 and step 2 are identical with embodiment 1;
[0043] Step 3, sample pre-wetting: put the four repeated test samples taken in step 2 into a beaker, and soak in water at 15°C-25°C for 8 hours;
[0044] Step 4, remove the exocarp mechanically: Press the pre-wetted seed sample in step 3 with tweezers, mechanically remove the exocarp, leave the endocarp and embryo, do not damage the embryo, for dryness and discoloration after taking the exocarp , moldy or empty seeds are treated as dead seeds, and the seed mortality rate is calculated;
[0045] Step 5, preparation of culture sand: Pass the river sand through a 0.05-0.8mm sieve, wash it with clean water, sterilize it at 140-145°C for 2 hours, and mix the sterilized sand with distilled water at a ratio of 90:10 (w...
Embodiment 3
[0050] A method for culturing in vitro embryos with testa in a rare woody economic crop sorbifolium sorbifolium seed viability measurement, the seed batch is from the Northwest sorbifolium sorbifolium base in Jingtai County, Gansu Province, and step 1 and step 2 are the same as in Example 1;
[0051] Step 3, sample pre-wetting: put the four repeated test samples taken in step 2 into a beaker, and soak in water at 15°C-25°C for 10 hours;
[0052] Step 4, mechanically remove the exocarp: press the pre-wetted seed sample in step 3 with tweezers, mechanically remove the exocarp, leave the endocarp and embryo, and do not damage the embryo. For shriveled, discolored, moldy or empty seeds after taking the outer testa, treat them as dead seeds, and calculate the seed mortality;
[0053] Step 5, preparation of the culture paper bed: soak the germination paper (Anchor paper, U.S.A.) in distilled water in the germination tray for 2-3 hours to saturate the germination bed with moisture; ...
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