Preparation method and product of avian influenza vaccine

A technology of bird flu and bird flu virus, which is applied in the field of preparation of live bird flu vaccine, can solve the problems of wasting the immune potential of the body, and achieve the effects of improving equipment utilization, good safety, and saving equipment space
CN109679900APending Publication Date: 2019-04-26哈药集团生物疫苗有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
哈药集团生物疫苗有限公司
Publication Date
2019-04-26

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Abstract

The invention discloses a preparation method of an avian influenza vaccine. The preparation method comprises the following steps: (1) carrying out acclimation culture on CEF (Chicken Embryo Fibroblast), thus obtaining sub-culture CEF which can adapt to a serum-free culture medium and is in a single-cell suspended growth state; (2) inoculating the sub-culture CEF obtained through acclimation in step (1) to a shake flask for carrying out shaking culture; (3) inoculating an avian influenza virus to the sub-culture CEF after the shaking culture, and carrying out propagation culture of the virus; (4) harvesting a virus solution; (5) preparing the avian influenza vaccine after deactivating the virus solution. According to the preparation method disclosed by the invention, the pollution risk andthe production cost are remarkably reduced, the production time of the avian influenza vaccine is effectively shortened, and the production scale can be conveniently amplified; the prepared avian influenza vaccine is good in safety and high in immunoprotection effect.
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Description

technical field

[0001] The invention relates to a preparation method of an avian influenza vaccine and a product thereof, in particular to a method for preparing an avian influenza vaccine by adopting a serum-free suspension culture technique and the obtained avian influenza vaccine product, and belongs to the field of preparation of a live avian influenza vaccine. Background technique

[0002] Most of the traditional production methods of avian influenza vaccines use the chicken embryo culture system, which is expensive to produce, and the residue may cause allergic reactions; and the chicken embryos used for vaccine production are planned supply, which cannot cope with large-scale outbreaks of avian influenza. Therefore, it is difficult to control the quality and quantity of the avian influenza vaccine produced by the chicken embryo culture system, and it is urgent to develop a continuous, stable, large-scale and economical and effective animal cell culture system to improv...

Claims

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