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Recombinant adenovirus, preparation method and application

A technology of recombinant adenovirus and adenovirus, which is applied in the fields of molecular biology and pharmacology, can solve problems such as balance disruption, and achieve the effect of high safety, high effectiveness, and less frequent administration

Inactive Publication Date: 2019-04-26
ANHUI MEDICAL UNIV
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  • Description
  • Claims
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Problems solved by technology

Under physiological conditions, apoptosis is strictly controlled by genes, however, under pathological conditions, the balance between cell proliferation and cell death is disrupted, causing a variety of liver diseases

Method used

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  • Recombinant adenovirus, preparation method and application
  • Recombinant adenovirus, preparation method and application
  • Recombinant adenovirus, preparation method and application

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Embodiment Construction

[0033] The following is a detailed description of the embodiments of the present invention. This embodiment is implemented on the premise of the technical solution of the present invention, and detailed implementation methods and specific operating procedures are provided, but the protection scope of the present invention is not limited to the following implementation example.

[0034] The specific implementation process of this embodiment is as follows:

[0035] 1. Animal model preparation and grouping

[0036] Four SD rats were randomly selected as the normal control group, and 16 SD rats were selected as the model group, and carbon tetrachloride (CCl 4 ) induction method to prepare liver fibrosis model. Rats in the model group were subcutaneously injected with 50% CCl4 (CCl4: peanut oil = 1:1, 1ml g -1 ), the normal group was injected with the same amount of peanut oil as a control in the same way, and the medicine was given once every two days for 8 weeks. After the ad...

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Abstract

The invention discloses recombinant adenovirus, a preparation method and application. The preparation method comprises the following steps: cloning a TMEM88 gene into a cloning region of adenovirus shuttle plasmids and constructing recombinant shuttle plasmids for expressing TMEM88; co-transforming the recombinant shuttle plasmids and adenovirus framework plasmids into host cells; carrying out homologous recombination to generate recombinant adenovirus plasmids; packaging through packaging cells to obtain adenovirus particles with infection activity and amplifying in the packaging cells; aftercollecting and cracking the packaging cells, purifying to obtain the recombinant adenovirus. According to the recombinant adenovirus, adenovirus is used as a carrier and is safe and reliable; the adenovirus is not combined with host chromosome DNA (Deoxyribonucleic Acid) and the risk of canceration is not caused; the recombinant adenovirus has a remarkable effect on hepatic fibrosis and the reversing effect can be observed with a single injection. The recombinant adenovirus is convenient for drug administration due to the drug administration manner; the recombinant adenovirus is introduced into rats by adopting a tail vein injection manner. The best time for reversing the hepatic fibrosis is obtained.

Description

technical field [0001] The invention relates to the fields of molecular biology and pharmacology, in particular to a recombinant adenovirus, a preparation method and an application. Background technique [0002] Liver fibrosis is a necessary stage for many chronic liver diseases to develop into liver cirrhosis. It is a pathological change caused by chronic liver damage caused by various reasons. It is characterized by excessive and abnormal deposition of extracellular matrix components in the liver and affects liver function. . When hepatic fibrosis occurs, fibrous septa are formed in the liver, which will destroy the normal intrahepatic microcirculation, reduce the intrahepatic vascular network, hinder the direct contact between blood flow and liver cells, and affect the material exchange between blood and liver cells, resulting in damage caused by inflammation. Damaged liver cells are not easy to repair or even aggravate the damage. Normal liver cells are hypoxic, degener...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/01C12N15/12C12N15/861A61K38/17A61K39/00A61P1/16
CPCA61K38/177A61K39/0005A61P1/16C12N7/00C12N15/86C12N2710/10021C12N2710/10043
Inventor 徐涛闫奇李良云周红胡爽潘林鑫孟晓明申传璞黄成张磊李俊
Owner ANHUI MEDICAL UNIV
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