Liquid chromatographic analysis method for detecting ziprasidone content in blood

A liquid chromatography analysis and ziprasidone technology, which is applied in the field of detecting the concentration of the antipsychotic drug ziprasidone in the blood, can solve the problems of complex ziprasidone drug concentration processing and poor method specificity, and reduce toxicity and side effects Response, improved accuracy, and reduced experimental cost

Inactive Publication Date: 2019-05-07
BEIJING HARMONY HEALTH MEDICAL DIAGNOSTICS CO LTD
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the invention of this application is to solve the problems of complex processing and poor method specificity in the existing detection of ziprasidone drug concentration in blood

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Liquid chromatographic analysis method for detecting ziprasidone content in blood
  • Liquid chromatographic analysis method for detecting ziprasidone content in blood
  • Liquid chromatographic analysis method for detecting ziprasidone content in blood

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0036] A liquid chromatography analysis method for detecting ziprasidone content in blood of the present invention, it comprises the following steps:

[0037] (1) Calibration of standard solution

[0038] (a) Preparation of standard working solution:

[0039]Accurately weigh 10mg of ziprasidone standard substance and place it in a 10mL volumetric flask, dissolve it with 10mL of methanol to obtain standard stock solution A, the concentration of its ziprasidone is 1000ug / mL, and use standard stock solution A with a water content of Dilute with 50% methanol solution, use ziprasidone solutions containing 300, 400, 800, 1600, 3200, 6400 and 12800ng / mL respectively as the standard working solution, and store at -80°C;

[0040] (b) Preparation of internal standard working solution

[0041] Accurately weigh 10 mg of verapamil standard substance in a 10 mL test tube, dissolve with 10 mL of methanol to obtain internal standard stock solution B, whose verapamil concentration is 1000 ug...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
wavelengthaaaaaaaaaa
emission peakaaaaaaaaaa
correlation coefficientaaaaaaaaaa
Login to view more

Abstract

The invention discloses a liquid chromatographic analysis method for detecting the ziprasidone content in blood. The method includes the following steps of: calibrating a standard solution by using ahigh performance liquid chromatograph and an FLD detector, fitting to obtain a standard curve equation of y=a*x+b, taking a to-be-detected blood sample, and after the to-be-detected blood is processed, detecting the to-be-detected sample by using the high performance liquid chromatograph and the FLD detector similarly to obtain a to-be-detected blood y value, substituting the to-be-detected bloody into the standard curve equation, and calculating the relative concentration x of ziprasidone in the to-be-detected blood sample. Since the concentration of the internal standard working fluid is known, the concentration of ziprasidone drugs in the to-be-detected blood is calculated. According to the scheme of the invention, the serum is subjected to sample injection after liquid-liquid extraction and concentration, the sample is detected by using the FLD detector, and the detection method has low detection limits and high specificity, and is more favorable for monitoring the blood concentration of ziprasidone in the bodies of patients during clinical treatment.

Description

technical field [0001] The invention relates to the technical field of clinical blood drug concentration monitoring of psychotropic drugs, in particular to a method for detecting the concentration of antipsychotic drug ziprasidone in blood. Background technique [0002] Schizophrenia is a clinical syndrome composed of a group of symptoms, and it is a multifactorial disease. Although the current understanding of its etiology is not very clear, the role of individual psychological susceptibility and adverse factors in the external social environment on the occurrence and development of the disease has been recognized by everyone. Both susceptibility quality and external adverse factors may cause the occurrence of the disease through the joint action of internal biological factors, and the pathogenesis factors of different patients may be more important in one aspect. [0003] Ziprasidone is an atypical antipsychotic that is structurally distinct from phenothiazine or butylpht...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/74
Inventor 张国成雒琴倪君君贾永娟
Owner BEIJING HARMONY HEALTH MEDICAL DIAGNOSTICS CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap