Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Chip for detecting common aquatic allergen antibodies and preparation method thereof

A technology for allergens and allergen proteins is applied in the field of chips for detecting the concentration of common aquatic allergen antibodies and their preparation, which can solve the problems of inconvenience to patients, high cost of reagents and instruments, and inability to detect software, etc. Simple detection and highly consistent detection rate

Inactive Publication Date: 2019-05-07
江苏三联生物工程股份有限公司
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the main product of German Minsie uses the membrane strip immunoblotting method, but none of its products can detect fish, crustaceans, and molluscs on the same membrane strip; Oumeng also uses the membrane strip immunoblotting method, It can only detect fish and crustaceans, but not molluscs; although Phadia has complete detection items, the cost of Phadia’s reagents and instruments is very high, which brings inconvenience to patients

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Chip for detecting common aquatic allergen antibodies and preparation method thereof
  • Chip for detecting common aquatic allergen antibodies and preparation method thereof
  • Chip for detecting common aquatic allergen antibodies and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A chip for detecting common allergens, the chip is prepared through the following steps:

[0035] (1) Black slide pretreatment;

[0036] ① Soak the black slide in the slide pretreatment solution containing NaOH for 16 hours, and then wash it with purified water for 2 to 8 times;

[0037] ② Soak black slides in 1 wt% silane solution (medium: 25% ethanol) for 20 min;

[0038] ③Purge the soaked black glass slides with nitrogen gas, put them in an oven, and bake them at 180°C for 0.2h.

[0039] (2) Spotting the allergen protein solution;

[0040] refer to figure 1 , using machine automation to spot samples, dotted into a 4×7 matrix on a black slide; each column is a dot formed by the same allergen protein solution; the allergen protein solution includes allergen proteins of fish, shrimp / crab, and mollusk solution.

[0041] (3) blocking allergen protein solution;

[0042] The spotted black slide was submerged in blocking solution (Tris buffer containing 3% bovine serum...

Embodiment 2

[0046] A chip for detecting common allergens, the chip is prepared through the following steps:

[0047] (1) Black slide pretreatment;

[0048] ① Soak the black slide in the slide pretreatment solution containing NaOH for 24 hours, and then wash it with purified water for 2 to 8 times;

[0049] ② Soak the black glass slide in a silane solution with a mass concentration of 0.05% by weight (the medium is 25% ethanol) for 60 minutes;

[0050] ③Purge the soaked black glass slides with nitrogen gas, put them in an oven, and bake them at 140°C for 0.4h.

[0051] (2) Spotting the allergen protein solution;

[0052] refer to figure 1 , using machine automation to spot samples, dotted into a 4×7 matrix on a black slide; each column is a dot formed by the same allergen protein solution; the allergen protein solution includes allergen proteins of fish, shrimp / crab, and mollusk solution.

[0053] (3) blocking allergen protein solution;

[0054] The spotted black slide was submerged ...

Embodiment 3

[0058] A chip for detecting common allergens, the chip is prepared through the following steps:

[0059] (1) Black slide pretreatment;

[0060] ① Soak the black slide in the slide pretreatment solution containing NaOH for 20 hours, and then wash it with purified water for 2 to 8 times;

[0061] ② Soak the black glass slide in a 0.5wt% silane solution (medium: 25% ethanol) for 30 minutes;

[0062] ③Purge the soaked black glass slides with nitrogen gas, put them into an oven, and bake them at 100°C for 0.6h.

[0063] (2) Spotting the allergen protein solution;

[0064] refer to figure 1 , using machine automation to spot samples, dotted into a 4×7 matrix on a black slide; each column is a dot formed by the same allergen protein solution; the allergen protein solution includes allergen proteins of fish, shrimp / crab, and mollusk solution.

[0065] (3) blocking allergen protein solution;

[0066] The spotted black slide was submerged in blocking solution (MOPS buffer containi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a protein chip capable of simultaneously detecting the concentration of common aquatic allergen antibodies. The chip is prepared by following steps: (1) black slide pretreatment; (2) sample application of an allergen protein solution; and (3) sealing of the chip to produce the chip. The chip disclosed by the invention is used for detecting the concentration of allergen antibodies in the blood of a patient, thereby judging whether or not the patient is allergic to an allergen to be tested and the degree of the allergen. A kit formed by the method is convenient to use, has high detection rate, and can also realize the automatic operation of allergen antibody detection.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a chip for detecting antibody concentration of common aquatic allergens and a preparation method thereof. Background technique [0002] With the rapid development of the global economy and the great changes in people's lifestyles and living environment conditions, the spectrum of diseases is also changing. At the World Health Assembly held by the World Health Organization in 2013, it was clearly stated that chronic non-communicable diseases (NCDs) represented by allergic diseases, due to the characteristics of early onset, fluctuating and repeated conditions, and complex and diverse clinical phenotypes, It has become a hot spot of global public health concern. [0003] Allergic disease, also known as allergic disease, refers to a kind of physiological dysfunction or tissue cell damage that occurs when the body is sensitized to some sensitizing substances in contact with it for the f...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/68G01N33/552G01N33/531
Inventor 汤迭尧泮锋纲丁俊杰施启尧
Owner 江苏三联生物工程股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com