A method for studying the acetylation sites of autophagy-related proteins in mature adipocytes

Abstract

The invention discloses a method for researching autophagy-related protein acetylation sites in mature adipocytes, which relates to the technical field of wine formulations, and includes the following steps: Step 1, confirming that there is acetylation modification in autophagy-related protein A, first culturing fat cells, and induced them to differentiate into mature adipocytes, and then treated with sirtuin inhibitors. The research method of autophagy-related protein acetylation sites in mature adipocytes is to identify acetylation sites on the basis of prediction and obtain the specific position of acetylation modification sites. By relocating and evaluating acetylation modification sites, The identification of acetylation modification sites is more accurate and credible, which can greatly improve the credibility of the search results. Then, the function research of acetylation sites is carried out, and the research process is the way of virus infection, so it can be used in To a certain extent, the time consumed by research experiments is shortened, and the method is reliable and has good repeatability.

Description

Technical field[0001]The invention relates to the technical field of wine formulations, in particular to a method for studying acetylation sites of autophagy-related proteins in mature adipocytes.Background technique[0002]Protein acetylation is the process of adding acetyl groups to protein lysine residues under the action of acetyltransferase. It is a mechanism for cells to control gene expression, protein activity or physiological processes.[0003]Acetylation is a highly regulated protein post-translational modification necessary to ensure protein activity. It can occur in core histones, nearly 40 transcription factors and more than 30 other protein targets, from bacteria to humans, protein acetylation It not only plays a key role in nuclear function, but also plays an important role in regulating various cytoplasmic metabolism, including cytoskeleton dynamics, energy metabolism, endocytosis and autophagy, and even includes transmembrane signal conduction. The recognition of dots w...

AI Technical Summary

Problems solved by technology

[0006] Aiming at the deficiencies of the prior art, the present invention provides a method for studying the acetylation sites of autophagy-related proteins in mature adipocytes, which solves the problem that most of the current research methods for the acetylation sites of autophagy-related proteins in adipocytes are directly on adipocytes. Staining treatment, and then observation through microscopes and other equipment, not only did not judge whether there is acetylation modification in fat cells, but also did not predict the acetylation site, resulting in too blind and easy to study the protein acetylation site. Waste valuable time of researchers while also reducing the issue of credibility of experimental results