A kind of preparation method of nicotine solution standard substance
A technology of nicotine solution and standard substance, applied in the field of tobacco products, can solve the problems of complex, high manufacturing cost and the like
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Embodiment 1
[0045] Example 1 Preparation of amino-modified cross-linked dextran gel filler
[0046] 1.1 Add 45% N,N-dimethylformamide to the reactor, then add 50% cross-linked dextran gel, heat up to 60±2°C, stir, reflux for 60 minutes, add 5% of 1,6-hexamethylene diisocyanate, stir evenly, and react at a constant temperature of 60±2°C for 8 hours. After the reaction is completed, it is washed with ethanol, taken out, and dried in a vacuum oven to obtain amino-modified cross-linked dextran gel filler.
[0047] 1.2 Using the above preparation method, 4-hydrazino-4-oxobutanoic acid was used instead of N,N-dimethylformamide to prepare amino-modified cross-linked dextran gel filler.
[0048] 1.3 Using the above preparation method, adipate dihydrazide was used instead of N,N-dimethylformamide to prepare amino-modified cross-linked dextran gel filler.
[0049] 1.4 Using the above preparation method, maleimide was used instead of N,N-dimethylformamide to prepare amino-modified cross-linked de...
Embodiment 2
[0054] Example 2 Preparation of standard substance of nicotine solution
[0055] 1) Preparation of the test product
[0056] Weigh 100g of tobacco leaf sample, add 2000ml of 5% sodium hydroxide solution, ultrasonically extract for 4h, then collect the filtrate by filtration, and concentrate the filtrate to about 10ml to form a nicotine aqueous solution as the test sample.
[0057] 2) Gel filtration column preparation
[0058] Take by weighing 500g of amino-modified cross-linked dextran gel filler (prepared by part 1.2 of Example 1), add 500ml of chromatographic grade methanol to swell for 15min, and pack the column (about 1.5 meters long) after the gel is fully swollen. The gel was washed alternately with deionized water and chromatographic grade methanol, and repeated 3 times.
[0059] 3) Gel filtration
[0060] The prepared nicotine aqueous solution is loaded, and ethanol and isopropanol (volume ratio is 1:2) are used as the mobile phase for gel filtration chromatograph...
Embodiment 3
[0070] Example 3 Preparation of standard substance of nicotine solution
[0071] 1) Preparation of the test product
[0072] Weigh 100g of tobacco leaf sample, add 3000ml of 5% sodium hydroxide solution, ultrasonically extract for 4h, then collect the filtrate by filtration, concentrate the filtrate to about 10ml, and form a nicotine aqueous solution as the test sample.
[0073] 2) Gel filtration column preparation
[0074] Take by weighing 500g of amino-modified cross-linked dextran gel filler (prepared by part 1.2 of Example 1), add 500ml of chromatographic grade methanol to swell for 30min, and pack the column (about 1.5 meters long) after the gel is fully swollen. The gel was washed alternately with deionized water and chromatographic grade methanol, and repeated 3 times.
[0075] 3) Gel filtration
[0076] The prepared nicotine aqueous solution is loaded, and ethanol and isopropanol (volume ratio is 1:4) are used as the mobile phase for gel filtration chromatography ...
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