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Application of ABHD6 in diagnosis, prognosis and treatment products of non-small cell lung cancer

A non-small cell lung cancer and prognosis technology, which is applied to the determination/test of microorganisms, medical preparations containing active ingredients, organic active ingredients, etc., can solve the problems of poor prognosis, unreported, low survival rate, etc.

Inactive Publication Date: 2019-05-14
谢昊
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some progress has been made in the treatment of lung cancer, more than half of lung cancer patients are already in the advanced stage or have distant metastasis when they are diagnosed, so the prognosis is still poor, with a median survival time of less than 18 months and a five-year survival rate of less than 15%
[0004] The expression level and mechanism of action of ABHD6 in NSCLC have not been reported in domestic and foreign literature

Method used

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  • Application of ABHD6 in diagnosis, prognosis and treatment products of non-small cell lung cancer
  • Application of ABHD6 in diagnosis, prognosis and treatment products of non-small cell lung cancer
  • Application of ABHD6 in diagnosis, prognosis and treatment products of non-small cell lung cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 The expression difference of ABHD6 in NSCLC cancer tissue and normal tissue

[0028] In this example, by extracting total RNA from NSCLC cancer tissues and normal tissues, real-time fluorescent quantitative PCR was used to detect the expression of ABHD6 and the internal reference gene GAPDH, and the differences in the expression of ABHD6 in NSCLC cancer tissues and normal tissues were analyzed. The specific method includes the following steps:

[0029] (1) NSCLC cancer tissue and normal tissue sample collection: Tissue samples from 206 NSCLC patients and 60 corresponding normal tissue samples adjacent to cancer were obtained from the Affiliated Hospital of Nantong University. The content of this experiment was approved by the Ethics Committee of the Affiliated Hospital of Nantong University. All cases are The patient's informed consent was obtained.

[0030] (2) Extract the total RNA of the tissue sample to be tested: add chloroform (1 / 5 the volume of RNAiso Plus), ...

Embodiment 2

[0039] Example 2 Statistical analysis of the relationship between ABHD6 expression level and clinical features and prognosis of lung cancer

[0040] We collected cancer tissue samples from 206 NSCLC patients and fabricated them into tissue chips. We used immunohistochemical methods to detect the expression level of ABHD6 in the tissues, and statistically analyzed the relationship between the expression of ABHD6 and clinicopathological parameters in 206 NSCLC patients. , And through COX single factor analysis, multivariate analysis and Kaplan-Meier survival curve to analyze the relationship between the expression of ABHD6 and prognosis.

[0041] The results show that ABHD6 protein is highly expressed in lung cancer tissues ( figure 2 ), high expression of ABHD6 is related to lymph node metastasis and TNM staging of lung cancer (Table 2); and is related to five-year survival rate ( image 3 , Table 3), is an independent factor for poor prognosis of lung cancer.

[0042] Table 2: The r...

Embodiment 3

[0049] Example 3 Silencing ABHD6 at the cellular level to study the role of ABHD6 in non-small cell lung cancer

[0050] The non-small cell lung cancer cell lines A549, SPC-A-1, NCI-H1675 and NCI-H1650 were respectively from the Cell Bank of the Chinese Academy of Sciences (CBTCCCAS, Shanghai). The NSCLC cells are stored in RPMI 1640 medium (Invitrogen). All media were supplemented with 10% fetal bovine serum (Thermo Fisher), 1% penicillin / streptomycin. All cells at 37°C, 5% CO 2 In the cultivation. First, Western blot was used to detect the expression level of ABHD6 in the four cells ( Figure 4 ).

[0051] Choose two cells A549 and SPC-A-1 with higher ABHD6 expression, use shRNA that silences ABHD6 or control shRNA to combine with pRNAU6.1 / NEO vector (control gene sequence is 5'-TTCTCCGAACGTGTCACGT-3'), and Four kinds of cells were transfected separately, and the cell's invasion and migration ability changes after silence were detected with Transwell chamber. The results show...

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Abstract

The invention discloses an application of ABHD6 in diagnosis, prognosis and treatment products of non-small cell lung cancer. The invention discloses a monoacylglycerol hydrolase ABHD6 (alpha / beta-hydrolase-domain-containing 6). The results of tissue microarray showed that the expression of ABHD6 in tumor tissues was significantly higher than that in adjacent tissues, and the high expression of ABHD6 was related to lymph node metastasis and TNM staging in non-small cell lung cancer, which was an independent factor of poor prognosis in NSCLC. The application of ABHD6 in diagnosis, prognosis andtreatment products of non-small cell lung cancer can influence the invasion and migration of non-small cell lung cancer cells in vitro by silencing ABHD6, and can slow down the growth speed of the tumor and slow down the process of the tumor migration to the lung.

Description

Technical field [0001] The invention belongs to the new application of ABHD6, and specifically relates to the application of ABHD6 gene in the preparation of non-small cell lung cancer diagnosis, treatment and prognosis detection products. Background technique [0002] Lung cancer is one of the most threatening malignant tumors to human health and life in the world. In my country, the incidence of lung cancer is increasing year by year, ranking the first cause of death from malignant tumors. Non-small cell lung cancer (NSCLC) It is the most common type of lung cancer. Although some progress has been made in the treatment of lung cancer, the prognosis is still poor because more than half of lung cancer patients are already at the advanced stage or the tumor has metastasized at the time of diagnosis. The median survival time is less than 18 months, and the five-year survival rate is lower than 15%. Early screening diagnosis is of great significance to the treatment of NSCLC and im...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886A61K45/00A61K31/7105A61P35/00
Inventor 汤志远谢昊郝海平
Owner 谢昊
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