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Multi-response fluorescence probe and preparation method thereof

A technology of fluorescent probes and tetrahydrofuran, which is applied in chemical instruments and methods, luminescent materials, organic chemistry, etc., can solve the troublesome and uncommon problems of preparation, and achieve the effects of simple preparation, rapid response, and low raw material cost

Inactive Publication Date: 2019-05-17
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the preparation of probes for the simultaneous detection of three or more analytes is uncommon because it requires the combination of multiple different responsive fluorescent units into the same fluorescent substance, which is very cumbersome to manufacture.

Method used

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  • Multi-response fluorescence probe and preparation method thereof
  • Multi-response fluorescence probe and preparation method thereof
  • Multi-response fluorescence probe and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Dissolve 0.5g of citric acid with 20ml of tetrahydrofuran under the condition of nitrogen gas, add dropwise to 10ml of tetrahydrofuran diluted 0.7ml of thionyl chloride solution, and react at -5°C for 12h. After the reaction is completed, steam excess thionyl chloride and citric acid chloride;

[0027] (2) Under nitrogen atmosphere, dissolve citric acid chloride with 20ml of tetrahydrofuran, then slowly add dropwise 0.4ml of N-aminoethylpiperazine diluted with 10ml of tetrahydrofuran, stir for 3h, add absolute ethanol, and vacuum the precipitate to obtain the final Product fluorescent probe.

Embodiment 2

[0029] (1) Dissolve 0.5g of citric acid with 20ml of tetrahydrofuran under the condition of nitrogen gas, add dropwise 1ml of thionyl chloride solution diluted with 10ml of tetrahydrofuran, and react at -10°C for 6h. After the reaction is completed, steam excess thionyl chloride and citric acid chloride;

[0030] (2) Under nitrogen atmosphere, dissolve citric acid chloride with 20ml of tetrahydrofuran, then slowly add dropwise 0.35ml of N-aminoethylpiperazine diluted with 10ml of tetrahydrofuran, stir for 3h, add absolute ethanol, vacuum the precipitate to obtain the final product Fluorescent probe.

Embodiment 3

[0032] (1) Dissolve 0.5g of citric acid with 20ml of tetrahydrofuran under nitrogen gas, add dropwise 0.5ml of thionyl chloride solution diluted with 10ml of tetrahydrofuran, and react at 10°C for 24h. After the reaction is completed, steam excess thionyl chloride and citric acid chloride;

[0033] (2) Under nitrogen atmosphere, dissolve citric acid chloride with 20ml tetrahydrofuran, then slowly add dropwise 0.6ml N-aminoethylpiperazine diluted with 10ml tetrahydrofuran, stir for 3h, add absolute ethanol, vacuum the precipitate to obtain the final product Fluorescent probe.

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Abstract

The invention discloses a multi-response fluorescence probe and a preparation method thereof. The structural formula of the fluorescence probe provided by the invention is shown as follows: (the formula is shown in the description). The invention further provides a preparation method of the fluorescence probe. The preparation method comprises the following steps that (1) citric acid and thionyl chloride have acylating chlorination under inert atmosphere to obtain citric aryl chloride; and (2) N-aminoethylpiperazine and citric aryl chloride have amidation under inert atmosphere to obtain the fluorescence probe. The fluorescence probe provided by the invention has good response to pH, the fluorescence intensity of the probe is linearly enhanced when PH is in the range of 10-12, and thus accurate testing of pH value is achieved; and mercury ions can be quantitatively and qualitatively detected when PH is 12, and glutathione is detected through weak interaction of sulfydryl mercury ions. In addition, the probe has the advantages of being easy and convenient to prepare, low in raw material cost, capable of rapidly and sensitively responding pH value, mercury ions and glutathione, and easy to popularize and apply.

Description

Technical field [0001] The invention relates to the technical field of fluorescent probes and their preparation, in particular to a multi-response fluorescent probe and a preparation method thereof. Background technique [0002] With the development of high-sensitivity and low-cost fluorescence sensing technology, fluorescent probes have become increasingly demanding in many fields and disciplines such as chemistry, biology and environmental science, and have gradually become an important research method in recent decades. Compared with other traditional detection methods, fluorescent probes have the characteristics of good selectivity, high sensitivity, simple operation, low cost, fast transient response, and low detection limit, which have attracted the attention of scientists. So far, it has been used to detect single analytes such as pH, Hg 2+ And GSH probes have been extensively studied. However, the preparation of probes for detecting three or more analytes at the same tim...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D295/13C09K11/06
Inventor 关瑞芳张昊
Owner UNIV OF JINAN