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A fluorescent aptamer probe based on Prussian blue nanoparticles and its preparation method and application

A nanoparticle, Prussian blue technology, applied in the field of biological nanotechnology sensing, to achieve the effect of improving low stability, low detection limit and strong signal

Active Publication Date: 2020-11-27
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there is no technology to construct fluorescent probes with Prussian blue nanoparticles and modified FAM fluorophore aptamers

Method used

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  • A fluorescent aptamer probe based on Prussian blue nanoparticles and its preparation method and application
  • A fluorescent aptamer probe based on Prussian blue nanoparticles and its preparation method and application
  • A fluorescent aptamer probe based on Prussian blue nanoparticles and its preparation method and application

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Embodiment 1

[0048] The preparation method of Prussian blue nanoparticles, the steps are as follows:

[0049] Take 20mL, 1mM K 4 [Fe(CN) 6 ] aqueous solution, heated to 60 ° C; dropwise added 20mL, 1mM Fe(NO 3 ) 3 Aqueous solution; the dropping process lasts about 10 minutes, and keep stirring. After 5 minutes, remove the heat source, keep stirring and cool to room temperature; the solution is alternately washed with water and ethanol three times, and then vacuum-dried to obtain the desired Prussian blue nanoparticles.

[0050] Nano fluorescent probe FAM-Apt Aβ The preparation method of @PBNPs, the steps are as follows:

[0051] Take 10 μL of FAM-Apt Aβ (500nM), 9μL PBNPs (1mg.mL -1 ) with 74μL HEPEs buffer (25mM, pH7.6, 100mM NaCl, 1mM MgCl 2 ) were mixed, incubated at 25° C. in the dark for 60 minutes, and 7 μL of BSA (10 μM) was added, and incubated for 60 minutes to obtain the nano fluorescent probe.

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Abstract

The invention discloses a fluorescent aptamer probe based on Prussian blue nanoparticles as well as a preparation method and application of the fluorescent aptamer probe. The fluorescent aptamer probeis composed of Prussian blue nanoparticle complex modified FAM fluorophore aptamers. The preparation method comprises the following steps: incubating the Prussian blue nanoparticles and the aptamersmodified with the FAM fluorophore in an HEPEs buffer solution system in a dark place, and closing by adopting BSA, thereby obtaining the fluorescent aptamer probe. The fluorescent aptamer probe can beused for detecting various markers such as markers of tumor, breast cancer, blood glucose and Alzheimer's disease, has the advantages of strong signal, high specificity, high sensitivity, wide detection concentration range, good biological safety and the like, and is beneficial to popularization and application.

Description

technical field [0001] The present invention relates to a fluorescent aptamer probe, in particular to a fluorescent aptamer probe for fluorescent detection of markers constructed based on the fluorescence quenching effect of Prussian blue nanoparticles on FAM fluorophores, and also relates to a The invention relates to a method for detecting various markers through changes in fluorescent signals, and belongs to the field of biological nanotechnology sensing. Background technique [0002] In recent years, with the development and research of nanotechnology, fluorescent aptasensors based on nanomaterials have received extensive attention. Various nanomaterials, such as gold nanoparticles (Hao W., Yongxiang W., Jianyu J., et al. Goldnanoparticle-based colorimetric and "turn-on" fluorescent probe for mercury(II)ions in aqueous solution[J], Analytical Chemistry, 2008, 80(23):9021-9028.), CeO 2 (Pautler R., Kelly E.Y., Huang P.J., et al.Attaching DNA to nanoceria:regulatingoxida...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/06C09K11/65C09K11/02G01N21/64
CPCC09K11/02C09K11/06C09K11/65G01N21/6428G01N2021/6432
Inventor 邓春艳陈文兰
Owner CENT SOUTH UNIV