Unlock instant, AI-driven research and patent intelligence for your innovation.

D-amino acid mixture medium and preparation method and application thereof

An amino acid, culture medium technology, applied in the field of microorganisms, can solve problems such as low Nisin yield

Inactive Publication Date: 2019-05-17
TIANJIN UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems existing in the prior art, the present invention provides a culture medium with D-amino acid mixture and its preparation method and application, so as to overcome the problem of low Nisin yield of Lactococcus lactis in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • D-amino acid mixture medium and preparation method and application thereof
  • D-amino acid mixture medium and preparation method and application thereof
  • D-amino acid mixture medium and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0029] The preparation method of adding D-amino acid mixture in the culture medium comprises the following steps:

[0030] (1) Weighing D-phenylalanine and D-methionine of corresponding quality respectively and adding them to sterile sodium bicarbonate solution (1M) preheated at 70°C to prepare mother solutions with respective concentrations of 0.8M;

[0031] (2) Heat in a water bath for about 20 minutes to dissolve, shake during heating to prevent agglomeration;

[0032] (3) After dissolving, pass the sterile water film on the ultra-clean bench while it is hot, and then add it into the sterile medium according to the concentration ratio range of 50mM:0mM~0mM:50mM, and shake well. A fermentation medium added with D-amino acid can be obtained.

[0033] Fermentation medium formula used in the present invention: yeast extract powder 15g / L, peptone 15g / L, potassium dihydrogen phosphate 20g / L, sucrose 15g / L, corn steep liquor 3g / L, cysteine ​​0.23g / L, chlorine Sodium chloride 1.5...

Embodiment 1

[0037] 1. Add the preparation method of D-amino acid mixture in the culture medium, comprise the following steps:

[0038] (1) Weighing D-phenylalanine and D-methionine of corresponding quality respectively and adding them to sterile sodium bicarbonate solution (1M) preheated at 70°C to prepare mother solutions with respective concentrations of 0.8M;

[0039] (2) Heat in a water bath for about 20 minutes to dissolve, shake during heating to prevent agglomeration;

[0040] (3) After dissolving, pass the sterile water film on the ultra-clean bench while it is hot, and then add it into the sterile medium according to the concentration ratio range of 50mM:0mM~0mM:50mM, and shake well. A fermentation medium added with D-amino acid can be obtained.

[0041] 2. Nisin production and acid stress of strain YF11

[0042] (1) Add the D-phenylalanine and D-methionine mother liquors into the sterilized fermentation medium in a ratio of 50:0, and shake well. The concentrations of the amin...

Embodiment 2

[0047] 1. Add the preparation method of D-amino acid mixture in the culture medium, comprise the following steps:

[0048] Repeat step 1 in the example

[0049] 2. Nisin production and acid stress of strain YF11

[0050] (1) D-phenylalanine and D-methionine mother liquor are added to the sterilized fermentation medium in a ratio of 0:50, and shake well. The concentrations of the amino acids are 0mM and 50mM respectively.

[0051] (2) Repeat (2) (3) in Step 2 of Example 1.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a D-amino acid mixture medium and a preparation method and application thereof. The method includes adding D-amino acid mixture to the medium to improve the acid resistance andthe nisin production of lactococcus lactis; fermenting an original strain for 12 h in the D-amino acid mixture medium. The Nisin yield reaches the maximum when the strain is cultured for 8h, the original strain is increased by 1.5-1.7 times, and the acid stress survival rate is increased by 1.4-1.7 times. The D-amino acid mixture has obvious influence on the product Nisin and the strain resistance, wherein the Nisin is a safe non-toxic food preservative, and the D-amino acid is widely used in food and medicine.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a culture medium added with D-amino acid mixture and its preparation method and application. Background technique [0002] D-amino acids are widely found in microorganisms, plants, and animals, and can be used in food, medicine, and cosmetics. The production and release of D-amino acids during cell growth regulates a variety of physiological changes, such as cell wall biosynthesis, biofilm integrity, and spore germination, among others. [0003] Lactococcus lactis is a prokaryotic microorganism that widely exists in dairy products and is widely used in the food industry. It is non-pathogenic to humans and animals and is recognized as a safe food-grade microorganism. Nisin is a polypeptide substance produced by Lactococcus lactis, consisting of 34 amino acid residues, with a molecular weight of about 3500Da, and can be absorbed by the human body. Nisin is a safe and non-toxic food ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20C12P21/02C12R1/01
Inventor 乔建军薛二淑吴昊财音青格乐宋倩倩田开仁支宁
Owner TIANJIN UNIV