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Molecular marker for detecting anti-scab QTL (quantitative trait loci) Qfhb.hbaas-5AS and application method of molecular marker

A technology for head blight and wheat head blight, applied in the field of molecular markers for detection of head blight resistance QTLQfhb.hbaas-5AS, can solve the problem of less application of gene breeding

Active Publication Date: 2019-05-24
INST OF FOOD CROPS HUBEI ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, except for Fhb1, the breeding applications of other genes are less reported

Method used

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  • Molecular marker for detecting anti-scab QTL (quantitative trait loci) Qfhb.hbaas-5AS and application method of molecular marker
  • Molecular marker for detecting anti-scab QTL (quantitative trait loci) Qfhb.hbaas-5AS and application method of molecular marker
  • Molecular marker for detecting anti-scab QTL (quantitative trait loci) Qfhb.hbaas-5AS and application method of molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1, Discovery of Qfhb.hbaas-5AS and its associated SNP markers

[0043] Test materials: WAPS (Wheat Association Panel for Scab research) population formed from 240 domestic and foreign wheat varieties (lines), see Table 1. The materials used are recorded in the literature: Zhu Wangwang, Xu Dengan, Cheng Shunhe, et al. Identification and traceability of the stripe rust resistance gene Fhb1 in Chinese wheat varieties [J]. Acta Crops, 2018, 44(4): 473–482. The public can download from Hubei Obtained from Food Crops Research Institute of Provincial Academy of Agricultural Sciences.

[0044] Table 1 Varieties (lines) and their origins of WAPS population

[0045]

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[0050]

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[0053]

[0054] a 90K SNP chip typing results; b Mark the detection results for KASP; c FHB index BLUE value (Best linear unbiased estimate) under 4 environments, "NN" means chip data missing.

[0055] 1. Discovery o...

Embodiment 2

[0099] Embodiment 2, actual sample detection

[0100] Test materials: WAPS species shown in Table 1.

[0101] 1. Identification of scab resistance

[0102] With embodiment 1.

[0103] 2. KASP-Fhb5AS marker detection

[0104] Mark with KASP-Fhb5AS according to the method in embodiment 1, carry out KASP reaction to 240 kinds shown in table 1, detect KASP reaction product; Use PHERAstar Plus Detect the product, and use KlusterCaller software for data analysis; if the color is red, the genotype of the wheat SNP site IWB21456 to be tested is AA; if it is blue, the gene of the wheat SNP site IWB21456 to be tested is AA; The type is GG.

[0105]The marker detection results of 240 wheat varieties and the average value of FHB index are shown in Table 1. The results in Table 1 were statistically analyzed to obtain the results in Table 4.

[0106] Table 4 Differences in scab resistance of different genotypes of KASP-Fhb5AS

[0107]

[0108] It can be seen from the above that th...

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Abstract

The invention discloses a molecular marker for detecting anti-scab QTL (quantitative trait loci) Qfhb.hbaas-5AS and an application method of the molecular marker. The invention further provides application of a substance for detecting the genotype of SNP IWB21456 loci of wheat chromosomes 5AS in identification or auxiliary identification of the resistance of the wheat scab resistance, and application of the substance for detecting the genotype of the SNP IWB21456 loci of the wheat chromosomes 5AS in preparation of products for identification or auxiliary identification of the wheat scab resistance. Through genome-wide association study (GWAS), the loci Qfhb.hbaas-5AS on short arms of the wheat chromosomes 5AS are discovered, and are further correlated with the SNP IWB21456 to be convertedinto common PCR markers KASP-Fhb5AS, and the concordance rate of the two for 240 materials is 96.7%. The molecular marker can be used for detecting the genotype of the anti-scab QTL Qfhb.hbaas-5AS, and for anti-scab molecular breeding.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a molecular marker for detecting scab resistance QTL Qfhb.hbaas-5AS and a method for using it. Background technique [0002] Fusarium head blight (FHB) caused by Fusarium graminearum is a widespread fungal disease that seriously affects wheat yield and quality. More importantly, the susceptible grains contain mycotoxins, such as deoxynivalenol (DON), which endanger the health of humans and animals and affect food and feeding. [0003] The middle and lower reaches of the Yangtze River and the Northeast wheat region in my country are areas where wheat scab frequently occurs and reoccurs. In recent years, affected by global climate change and the popularity of straw returning under the wheat-corn rotation system, the disease has shown a trend of expansion and aggravation, and has become a common disease in the Huanghuai wheat region. At present, the average annual occurrence area of ​...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
Inventor 朱展望付路平陈延平刘易科何伟杰贾梦洁郝元峰高春保夏先春何中虎
Owner INST OF FOOD CROPS HUBEI ACAD OF AGRI SCI
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