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A preparation method of gelatin methacrylamide core-shell microspheres for 3D cell culture

A technology of gelatin methacrylamide and core-shell microspheres, which can be used in the preparation of microspheres, animal cells, and microcapsule preparations, and can solve the problems of particle size controllability and poor uniformity, poor controllability, and poor uniformity

Active Publication Date: 2021-09-17
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The traditional microsphere preparation methods are mainly hanging drop method and mechanical stirring method. Due to the problems of poor controllability and poor uniformity, it is limited in the aspect of cell 3D culture. Subsequently, the new technology of microporous membrane was proposed, although the particle size of microspheres The controllability and uniformity of the particle size are very good, but the controllability and uniformity of the particle size are relatively poor in a small system

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  • A preparation method of gelatin methacrylamide core-shell microspheres for 3D cell culture
  • A preparation method of gelatin methacrylamide core-shell microspheres for 3D cell culture
  • A preparation method of gelatin methacrylamide core-shell microspheres for 3D cell culture

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Embodiment 1

[0028] A kind of microfluidic chip of the present invention, such as figure 1 As shown, it is mainly composed of continuous phase inlet 1, shell fluid inlet 2, core fluid inlet 3, microsphere outlet 4, continuous phase channel 5, shell fluid channel 6, core fluid channel 7, laminar flow channel 8 and main channel 9; The continuous phase inlet 1 is connected to the main channel 8 through the continuous phase channel 5, and the shell fluid inlet 2 and the core fluid inlet 3 are respectively connected to the laminar flow channel 8 and the main channel 9 through the shell fluid channel 6 and the core fluid channel 7;

[0029] In the microfluidic chip, the height and width of the continuous phase channel are 310 μm and 270 μm respectively, the height and width of the shell fluid channel are 150 μm and 150 μm respectively, the height and width of the core fluid channel are 150 μm and 130 μm respectively, and the height and width of the laminar flow channel are They are 150 μm and 15...

Embodiment 2

[0041] A kind of microfluidic chip of the present invention, such as figure 1 As shown, it is mainly composed of continuous phase inlet 1, shell fluid inlet 2, core fluid inlet 3, microsphere outlet 4, continuous phase channel 5, shell fluid channel 6, core fluid channel 7, laminar flow channel 8 and main channel 9; The continuous phase inlet 1 is connected to the main channel 8 through the continuous phase channel 5, and the shell fluid inlet 2 and the core fluid inlet 3 are respectively connected to the laminar flow channel 8 and the main channel 9 through the shell fluid channel 6 and the core fluid channel 7;

[0042] In the microfluidic chip, the height and width of the continuous phase channel 5 are 310 μm and 270 μm respectively, the height and width of the shell fluid channel 6 are 150 μm and 150 μm respectively, the height and width of the core fluid channel 7 are 150 μm and 130 μm respectively, and the laminar flow channel The height and width of 8 are 150 μm and 150...

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Abstract

The invention provides a method for preparing 3D cell cultured gelatin methacrylamide core-shell microspheres. The method mainly includes: synthesis of gelatin methacrylamide material, cell loading in gelatin methacrylamide core-shell microsphere core, 3D single / co-cultivation of cells, and the like. The invention achieves one-step, controllable, preparation of core-shell microspheres, and is used for cell 3D culture. Based on the good biocompatibility of the material, the invention is useful in cell culture, microtissue model construction, tissue block transplantation, drug slow release and screening. It has great application value in biological applications.

Description

technical field [0001] The invention relates to the technical fields of material chemistry and microfluidics, in particular to a method for preparing 3D cell-cultured gelatin methacrylamide core-shell microspheres. Background technique [0002] The construction of functional tissue models in vitro has been widely concerned in the fields of tissue engineering, tissue block transplantation, wound healing, drug screening and so on. In vivo, functionalizing tissues involves multiple types of cells and their interactions with the extracellular matrix. In addition, in the construction of functionalized tissues in vitro, the extracellular matrix also provides a 3D scaffold material and is used to achieve the cultivation of one or more types of cells. [0003] As a scaffold material for 3D cell culture, hydrogel materials have been widely used in cell culture and tissue block transplantation. The currently used hydrogel materials for 3D cell culture are mainly divided into two typ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00B01J13/02B01J13/14B01J13/20C12N11/12C12N11/04C12N5/09C12N5/071C08F289/00C08F220/56
Inventor 秦建华王慧刘海涛
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI