Silver ion detection method, silver ion detection kit and aptamer applied to detecting silver ions

Abstract

The invention relates to a silver ion detection method, a silver ion detection kit and an aptamer applied to detecting silver ions. The method comprises the following steps: providing a sample to be tested; mixing the sample to be tested with oxidized graphene, double-strand specific exonuclease and the aptamer with a fluorescent label to obtain a mixture, wherein the aptamer has a nucleotide sequence shown as SEQ ID NO.1; incubating the mixture for 5 to 40 min under the condition of 35 to 40 DEG C and performing inactivating treatment to inactivate the double-strand specific exonuclease; detecting the fluorescence intensity of the mixture which is inactivated and determining the concentration of silver ions in the sample to be tested. According to the silver ion detection method disclosedby the invention, high-selectivity and high-flexibility detection of the target silver ions can be achieved only through the aptamer with the fluorescent label, the oxidized graphene, the double-strand specific exonuclease and fluorescent detection equipment; an operation process of the silver ion detection method is simple and quick.

Description

technical field [0001] The invention relates to the field of silver ion detection, in particular to a silver ion detection method, a silver ion detection kit and a nucleic acid aptamer for detecting silver ions. Background technique [0002] Silver (Ag) is widely used in chemical, chemical, photosensitive, electronic and electrical industries due to its good bactericidal, catalytic, optical and electrical properties. In the production and application process of these industries, industrial wastewater containing a large amount of silver ions will be discharged into the environment, making silver ions one of the most polluting heavy metals. Silver ions can enter organisms through the enrichment of the food chain. Long-term exposure of organisms to high-concentration silver ions will inhibit the activity of proteins in organisms and cause certain damage to the health of organisms. At present, traditional silver ion detection methods include chemical methods, electrochemical an...

AI Technical Summary

Problems solved by technology

At present, traditional silver ion detection methods include chemical methods, electrochemical analysis methods, atomic absorption spectrometry, inductively coupled plasma mass spectrometry, etc., but these technologies have their own limitations, such as complexometric titration in chemical methods There are difficulties in the detection of trace substances; although the electrochemical analysis method has high sensitivity and wide linear range, its selectivity is poor; although atomic absorption spectroscopy and inductively coupled plasma mass spectrometry have relatively high detection accuracy, the equipment is expensive and the operating cost is high , There are many operating requirements, the detection sensitivity is low, the detection is time-consuming and laborious, and complex pre-treatment processes such as extraction, concentration and enrichment, or suppression of interference are required

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