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Composition containing intravaginal microorganisms

A technology of microorganisms and compositions, which is applied in the field of compositions containing vaginal microorganisms, and can solve problems such as female vaginal microflora that have not been reported yet

Inactive Publication Date: 2019-06-04
SEOUL NAT UNIV R&DB FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To date, studies of the disease and genetic influences on the human microbiome have been limited to the gut microbiome, but the female vaginal microbiome has not been reported

Method used

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  • Composition containing intravaginal microorganisms
  • Composition containing intravaginal microorganisms
  • Composition containing intravaginal microorganisms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Example 1. Research subjects and sample collection

[0071] For the present invention, endocervix samples were collected by brush from 542 female identical tweens sand fraternal twins and their families in the tween cohorts in Korea and frozen at -80°C. state storage. Transfer cryopreserved samples to the laboratory and use a kit to extract genomic DNA.

Embodiment 2

[0072] Example 2. Using 16S rRNA to analyze the vaginal microbial community

[0073] Genomic DNA extracted from Example 1 was amplified using the 515F / 806R primers (SEQ ID NO: 1 and 2) shown in Table 1, targeting the V4 region of the bacteria, and then the nucleotide sequence was obtained using Illumina's MiSeq instrument data.

[0074] [Table 2]

[0075]

[0076] By utilizing the QIIME pipeline, nucleotide sequence data were analyzed to determine genetic information and vaginal microbiome structure, and then to observe the relationship of the vaginal microbiome to various markers of women's health.

[0077] Vaginal microbiota information obtained by 16S rRNA-based analysis confirmed changes in vaginal microbiota according to menopausal status, vaginitis, cervicitis, cervical cancer, and obesity. The results are shown in figure 1 middle. As a result of analyzing factor associations, it was confirmed that the clinical indicators that can significantly change the structur...

Embodiment 3

[0079] Example 3, Analysis of the Correlation Between Vaginal Microflora and Female Genital Diseases or Obesity

[0080] The results of multivariate analysis using MaAsLin software, which can control for confounding variables, clarified female genital disorders or obesity, etc., identified by vaginal bacteria.

[0081] Using MaAsLin software, twin and family relationship were assigned as random variables and other health factors as calibration variables, and the correlation between each health factor and vaginal microbiota was analyzed. The results are shown in Figures 4 to 8 middle.

[0082] From Figure 4 , 5 As can be seen in and 8, Prevotella spp. was found to be most associated with female genital disorders, especially menopause, vaginitis and cervical cancer. In the case of obesity, P. copri, a species of Prevotella spp., was found to be the second highest correlate of obesity ( Figure 6 ).

[0083] In addition to postmenopausal status, vaginitis infection, and o...

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Abstract

The present invention relates to: a marker enabling the prediction or the diagnosis of risks of female genital organ diseases or obesity; and a use thereof. More specifically, the present invention relates to: a composition for predicting or diagnosing risks of female genital organ diseases or obesity by detecting, in a specimen sample, a Prevotella sp. strain, a Sneathia sp. strain, a Megasphaerasp. strain, a Gardnerella sp. strain and a Lactobacillus sp. strain, which are in the vagina; a kit; and a diagnostic method.

Description

technical field [0001] The present invention relates to the screening and use of biomarkers for predicting or diagnosing risk of female genital diseases or obesity by utilizing vaginal microbes. Background technique [0002] Worldwide, three quarters of the female population are experiencing infections from inflammatory female diseases, including bacterial vaginosis, of which 50% are reported to be recurrent. In addition, more than 60% of women with vaginitis are asymptomatic infections, and susceptibility to vaginitis is greatly increased due to antibiotics, stress, and postmenopausal hormonal changes. [0003] The causes of vaginitis due to infection fall into three main categories: bacterial vaginosis (BV), candidal vaginitis (CV), and Trichomonas vaginalis. Bacterial vaginosis is the most common vaginitis in recent years. According to reports, the vaginal secretions of patients with bacterial vaginosis include Atopobium vaginae, Megasphaera spp., Gardnerella vaginalis ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883G01N33/50G01N33/569
CPCG01N33/56911G01N2800/044G01N2800/36G01N2800/50C12Q1/04G01N2333/335C12Q1/689C12Q1/6883G01N33/5082G01N2500/04C12Q2600/112C12Q2600/136C12Q2600/118G01N33/502
Inventor 高光杓柴智娟柳炫朱
Owner SEOUL NAT UNIV R&DB FOUND
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