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A preparation method of liquid strain of Flammulina velutipes containing a high number of mycelial growth points

A technology for liquid strains and Flammulina velutipes, which is applied in the fields of edible fungi and bioengineering applications, can solve the problems of few mycelial growth points, cumbersome preparation process, economy and pollution, etc., and achieves the promotion of mycelium growth, less preparation process and synchronization. good effect

Active Publication Date: 2021-05-11
NINGDE NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

From the perspective of economy and pollution risk, the preparation process of the above-mentioned liquid strains is quite cumbersome, and the cycle usually takes more than 10 days. In addition, the preparation and cultivation process is extremely prone to pollution
On the other hand, during the liquid culture of Flammulina velutipes, mycelium aggregates and mycelium balls are mainly formed. The traditional seed tank culture mainly adopts the low-speed stirring method for cultivation. At the end of the culture, the liquid bacteria mainly exist in the form of mycelium balls. Therefore, There are very few mycelial growth points when the liquid strain is inserted into the cultivation material, and it is usually necessary to increase the inoculation amount, and the inoculation is inconvenient and unfavorable for dispersing into the culture material. method is important

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Prepare powdered Flammulina velutipes solid strain: crush the sawdust and pass through a 20-mesh sieve;

[0033] 2. Mix 80 parts of sawdust powder, 18 parts of soybean flour and 2 parts of soybean oil, adjust the water content to 50%, put 200 g of wet material into a 500 mL triangular flask, compact it slightly, and seal it with a rubber stopper;

[0034] 3. Sterilize the compost at 121°C for 2 hours to ensure complete sterilization;

[0035] 4. After cooling, insert a piece of agar block bacteria about 2 cm long and 1 cm wide cut from the slanted parent species of Flammulina velutipes under aseptic conditions to the surface of the culture material, in a dark environment at 25°C and relative humidity of 60-70% Medium culture, the mycelium can be used as the inoculum for the production of liquid strains in the seed tank after the mycelium is completely fed;

[0036] 5. Disinfection of seed tanks: use 100 L seed tanks, sterilize at 121°C for 30 minutes; add 70 L of l...

Embodiment 2

[0043] 1. The preparation of powdery Flammulina velutipes solid strain is the same as in Example 1.

[0044] 2. Store the solid strain of Flammulina velutipes at 4°C for 1 month before use.

[0045] 3. The size of the seed tank, disinfection and inoculation are the same as in Example 1.

[0046] 4. Culture conditions, temperature 25°C, ventilation rate 0.5 vvm, agitation conditions: 400 rev / min on the first day (to fully disperse the inserted solid bacteria), no agitation in the next 2-4 days (use aeration to make the culture Stirring), which can save power consumption, the rotation speed is set to 400 rev / min from the 5th day (using strong shear force to disperse and cut the mycelial balls and mycelium to significantly increase the growth point of mycelium), 6 h, and then Stirring was stopped at 6 h to rejuvenate the mycelia, and the detection showed that the activity of the mycelia was similar to that before breaking and was in a state of high activity.

[0047] 5. The liq...

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Abstract

The invention discloses a method for preparing a liquid strain of Flammulina velutipes containing a high number of mycelium growth points. The method comprises the following steps: 1. using the powdered culture material matrix as raw material to prepare the solid strain of Flammulina velutipes as an inoculum; The present invention utilizes powdered solid strains as the inoculum produced by the liquid strains of Flammulina velutipes, and its preparation method is simple, convenient and can be used for a long time; the seed tank culture adopts a special stirring method, that is, after the powdery strains are inserted, the high The stirring speed is to efficiently disperse the solid bacteria, increase the growth point, and then stop the stirring to save power consumption. In the later stage of cultivation, stir at a high speed for a short time to fully break up the mycelium and increase the growth point of the mycelium. Finally, cultivate for 6 hours to restore activity ; Adjust the pH of the seed solution to 8‑9 before use, which is beneficial to inhibit the growth of bacteria after inoculation. Adopting the method of the invention to prepare the liquid strain of Flammulina velutipes has great application prospects.

Description

technical field [0001] The invention belongs to the field of edible fungi and bioengineering applications, and relates to a method for preparing a liquid strain of Flammulina velutipes containing a high number of mycelium growth points. Background technique [0002] Flammulina velutipes ( Flammulina velutipes ), also known as mushrooms, mushrooms, etc., is a famous edible fungus in the world, which is widely cultivated in my country, Japan, South Korea and other countries. Flammulina velutipes is delicious and nutritious. It is a natural food with high protein, low calorie and high polysaccharide content. Flammulina velutipes contains 18 kinds of amino acids, among which the content of arginine and lysine is higher than that of common edible fungi. Therefore, Flammulina velutipes has a good effect on promoting the growth and development of teenagers, and is known as "wisdom-enhancing mushroom". In addition, Flammulina velutipes also has high medicinal value, such as loweri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G18/40A01G18/20A01G18/50A01G18/00
Inventor 刘盛荣张维瑞郑世仲阮俊峰
Owner NINGDE NORMAL UNIV
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