Composition capable of inducing stem cells to secrete cytokines and its application

A technology of cytokines and mesenchymal stem cells, applied in the field of compositions capable of inducing stem cells to secrete cytokines, can solve problems such as undisclosed induction compositions, and achieve the effects of avoiding social and ethical disputes, low immune rejection, and fast proliferation

Active Publication Date: 2021-02-26
GUANGZHOU RAINHOME PHARM&TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Traditional technology stimulates stem cells to secrete cytokines by adding inducing components to the stem cell culture medium, but there is no inducing composition disclosed, which can be well used to stimulate umbilical cord mesenchymal stem cells to secrete cytokines

Method used

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  • Composition capable of inducing stem cells to secrete cytokines and its application
  • Composition capable of inducing stem cells to secrete cytokines and its application

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Experimental program
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Effect test

Embodiment 1

[0034] This embodiment provides a composition capable of inducing stem cells to secrete cytokines and its application. Specifically, the composition is added to high-sugar DMEM to prepare a culture medium for inducing umbilical cord mesenchymal stem cells to secrete cytokines, and to stimulate umbilical cord mesenchymal stem cells to secrete cytokines.

[0035] 1. Medium for inducing umbilical cord mesenchymal stem cells to secrete cytokines (see Table 1): 100 mL of high-sugar DMEM, 3 mg of VA, 75 mg of glutamine, 1 mg of lysophosphatidic acid, and 0.05 mg of icariin.

[0036] 2. The method of inducing umbilical cord mesenchymal stem cells to secrete cytokines:

[0037] (1) Take the umbilical cord mesenchymal stem cells, culture them in the umbilical cord mesenchymal stem cell medium, and when the cells grow to about 85%, continue to subculture in the umbilical cord mesenchymal stem cell medium;

[0038] (2) Transfer the P3-P5 cells to the mechanical stretching cell culture b...

Embodiment 2

[0043] This embodiment is a modification example of Embodiment 1, and the changes relative to Embodiment 1 include:

[0044] 1. Medium for inducing umbilical cord mesenchymal stem cells to secrete cytokines (see Table 1): 100 mL high-glucose DMEM, 1 mg VA, 50 mg glutamine, 0.5 mg lysophosphatidic acid, and 0.01 mg icariin.

[0045] 2. In step (2): Transfer P3-P5 cells to the mechanically stretched cell culture box. When the cell growth density reaches 50%-65%, discard the original umbilical cord mesenchymal stem cell culture medium and wash with PBS for three times. Replace with the culture medium that induces umbilical cord mesenchymal stem cells to secrete cytokines. After culturing for 1 hour, stretch the elastic membrane by 5% every 10 hours according to the scale on the wall of the mechanical pulling culture box, and place it in a carbon dioxide incubator. Cultivation was continued for 24 hours; other operations were the same as in Example 1.

Embodiment 3

[0047] This embodiment is a modification example of Embodiment 1, and the changes relative to Embodiment 1 include:

[0048] 1. Medium for inducing umbilical cord mesenchymal stem cells to secrete cytokines (see Table 1): 100 mL of high-sugar DMEM, 5 mg of VA, 100 mg of glutamine, 2 mg of lysophosphatidic acid, and 0.1 mg of icariin.

[0049] 2. In step (2): Transfer P3-P5 cells to the mechanically stretched cell culture box. When the cell growth density reaches 50%-65%, discard the original umbilical cord mesenchymal stem cell culture medium and wash with PBS for three times. Replace with the medium that induces umbilical cord mesenchymal stem cells to secrete cytokines for culture. After 3 hours of culture, stretch the elastic membrane by 15% every 15 hours according to the scale on the wall of the mechanical pulling culture box, and place it in a carbon dioxide incubator. Cultivation was continued for 48 hours; other operations were the same as in Example 1.

[0050] Table...

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Abstract

The invention relates to a composition capable of inducing stem cells to secrete cytokines and its application. The composition comprises: 1mg-5mg vitamin A, 50mg-100mg glutamine, 0.5mg-2mg lysophosphatidic acid, 0.01mg-0.1mg icariin. The composition is added to the induction medium formed in high-sugar DMEM, and the induction medium can stimulate the umbilical cord mesenchymal stem cells to secrete cytokines. In particular, in the process of induction culture, combined with appropriate mechanical stretching and physical stimulation, the amount of cytokines secreted by umbilical cord mesenchymal stem cells can be further enhanced. In addition, the present invention uses umbilical cord mesenchymal stem cells to avoid social and ethical disputes, with faster proliferation and low immune rejection.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a composition capable of inducing stem cells to secrete cytokines and an application thereof. Background technique [0002] Mesenchymal stem cells are a kind of pluripotent stem cells with self-renewal ability, which widely exist in a variety of adult tissues or perinatal tissues, such as bone marrow, fat, dental pulp, placenta, umbilical cord, etc. Umbilical cord mesenchymal stem cells are more convenient to extract and easier to obtain. In the application of regenerative medicine, umbilical cord mesenchymal stem cells have stronger effects of inhibiting endothelial cells and tissue apoptosis, and regulating immunity, and these effects are very closely related to the compound cytokines they secrete. During the culture process of umbilical cord mesenchymal stem cells, a variety of cytokines are secreted, such as epidermal growth factor (Epidermal growth factor, EGF), bas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0775C12P21/02
Inventor 黄燕飞车七石刘少辉
Owner GUANGZHOU RAINHOME PHARM&TECH CO LTD
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