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A method for processing immunohistochemical images

A technology of immunohistochemistry and image processing, which is applied in image data processing, image analysis, instruments, etc., can solve the problems of not clear enough images, fading, and the influence of image processing results, and achieve clear images, easy identification, and prolong the fading time. Effect

Active Publication Date: 2022-04-12
上海启肽智能科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the prior art, the dyeing effect is not good, resulting in the extracted image not being clear enough, which has a certain impact on the image processing results. Moreover, the fading phenomenon occurs in a short period of time after dyeing, which affects the extraction and observation of the secondary image

Method used

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  • A method for processing immunohistochemical images
  • A method for processing immunohistochemical images
  • A method for processing immunohistochemical images

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Experimental program
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Embodiment 1

[0028] A method for processing immunohistochemical images, comprising the following steps:

[0029] (a) Perform full-stained immunohistochemical staining on tissue sections to obtain digital immunohistochemical images; wherein, the staining process is: after mixing DAB staining solution with potassium dihydrogen phosphate, gelatin, and acetic acid (mass ratio: 100:0.2 :0.03:0.06) to stain the positive product, rinse with sodium dihydrogen phosphate solution (concentration of sodium dihydrogen phosphate solution is 0.2g / L) for 2 to 3 minutes, rinse with water, and mix hematoxylin staining solution with glycerol and betaine (Mass ratio: 100:0.1:0.03) After counterstaining cell nuclei, hydrochloric acid alcohol differentiation, water washing, dehydration, transparent, mounting, microscopic examination;

[0030] (b) convert the immunohistochemical image described in (a) step from RGB color space to YIQ color space, and normalize the value of the Y component of the transformed imag...

Embodiment 2

[0040] A method for processing immunohistochemical images, comprising the following steps:

[0041] (a) Perform full-staining immunohistochemical staining on tissue sections to obtain digital immunohistochemical images; wherein, the staining process is: mixing DAB staining solution with potassium dihydrogen phosphate, gelatin, and acetic acid (mass ratio: 100:0.1: 0.02:0.05) to stain the positive product, wash with sodium dihydrogen phosphate solution (the concentration of sodium dihydrogen phosphate solution is 0.2g / L) for 2 to 3 minutes, rinse with water, and mix hematoxylin staining solution with glycerol and betaine (Mass ratio: 100:0.2:0.05) After counterstaining cell nuclei, hydrochloric acid alcohol differentiation, water washing, dehydration, transparent, mounting, microscopic examination;

[0042] (b) convert the immunohistochemical image described in (a) step from RGB color space to YIQ color space, and normalize the value of the Y component of the transformed image ...

Embodiment 3

[0051] A method for processing immunohistochemical images, comprising the following steps:

[0052] (a) Perform full-staining immunohistochemical staining on tissue sections to obtain digital immunohistochemical images; wherein, the staining process is: mixing DAB staining solution with potassium dihydrogen phosphate, gelatin, and acetic acid (mass ratio: 100:0.2: 0.05:0.1) After staining the positive product, rinse with sodium dihydrogen phosphate solution (concentration of sodium dihydrogen phosphate solution is 0.3g / L) for 2 to 3 minutes, rinse with water, and mix hematoxylin staining solution with glycerol and betaine (Mass ratio: 100:0.2:0.05) After counterstaining cell nuclei, hydrochloric acid alcohol differentiation, water washing, dehydration, transparent, mounting, microscopic examination;

[0053] (b) convert the immunohistochemical image described in (a) step from RGB color space to YIQ color space, and normalize the value of the Y component of the transformed imag...

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Abstract

The present invention provides a method for processing immunohistochemical images, comprising the following steps: (a) staining to obtain immunohistochemical images; (b) converting the immunohistochemical images to YIQ color space; (c) determining the brightness threshold T1; ( d) Carry out image segmentation; (e) convert the immunohistochemical image to HSV color space; (f) extract the intensity value of the pixel in the nucleus area; (g) determine the recognition threshold T2; (h) divide the pixel in the nucleus area into There are two classes of Class1 and Class2; (i) automatically label the positive and negative categories of the two classes of pixels of Class1 and Class2. The image processing method provided by the present invention mainly optimizes the dyeing process. Adding potassium dihydrogen phosphate, gelatin, acetic acid, glycerin and betaine makes the image clearer, easier to identify and the cells are not easy to fade, and the washing with sodium dihydrogen phosphate solution plays an auxiliary role. Effect, prolong the fading time.

Description

technical field [0001] The invention relates to the technical field of cell observation, in particular to an immunohistochemical image processing method. Background technique [0002] Immunohistochemical technology refers to the technology of qualitative, localization and quantitative determination of the corresponding antigen by using the specific binding reaction between the antigen and the antibody in the principle of immunology by binding the antibody to the chromogenic agent. important method. After immunohistochemical staining, cells or tissues are imaged under a microscope to obtain color images, which carry important information for the design of immunohistochemical experiments. The image data can be obtained by identifying, segmenting, and processing the image, so as to accurately judge the results of immunohistochemistry, and clear and easily identifiable images are the prerequisite for obtaining read data information. In the prior art, the dyeing effect is poor,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/532G01N33/558G06T7/136G06T7/90
Inventor 鲁南马立新齐国光
Owner 上海启肽智能科技有限公司
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