Method for eliminating flatulence of biogas slurry agricultural bio-fertilizer soft packaging and improving fertilizer efficiency
A bio-fertilizer and flexible packaging technology, applied in fertilization devices, fertilizer mixtures, applications, etc., can solve the problems of product storage, transportation, and sales, hidden safety hazards, intensified microbial fermentation process, and flatulence of biogas slurry fertilizer soft film bags, etc., to improve The ability to resist diseases, promote the germination rate of crops, and eliminate the effect of high temperature flatulence
Pending Publication Date: 2019-06-11
YANCHENG TEACHERS UNIV
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Problems solved by technology
Commercial concentrated biogas slurry fertilizers are often filled with composite soft film bags to reduce product packaging costs, but as the temperature rises, the microbial fermentation process in biogas slurry fertilizers intensifies, and the soft film bags of biogas slurry fertilizers are severely inflated and even broken. Not only caused huge economic losses, but also had potential safety hazards in product storage, transportation, and sales
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[0033] Strain activation medium (LB medium): Add 10g tryptone, 5g yeast extract, and 10g NaCl to 950ml deionized water, shake the container until the solute is dissolved, adjust the pH to 7.0 with 1mol / L NaOH, and dilute to 1L with deionized water , steam sterilized at 15psi high pressure for 20min.
[0034] Activation of Bacillus pumilus WP8 strain: transfer the strain to LB solid plate, incubate upside down at 30°C for 18-24 hours, pick a single colony and transfer it to LB liquid medium, shake and culture at 30°C, 120-150rpm for 1 8-24 hours, then prepare seeds liquid. Transfer to LB liquid medium with 1% inoculum amount, shake culture at 30°C for 18-24h, transfer to 4°C and let stand for 30min to prepare bacterial suspension.
[0035] Activation of Pseudomonasputida sp.RBP1 strain: transfer the strain to LB solid plate, invert culture at 30°C for 18-24 hours, pick a single colony and transfer it to LB liquid medium, 30°C, 120-150rpm shaking culture for 18-24 hours, then p...
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The invention discloses a method for eliminating flatulence of biogas slurry agricultural bio-fertilizer soft packaging and improving fertilizer efficiency. A composite microbial agent and CaO2 are added to a biogas slurry agricultural bio-fertilizer, and the composite microbial agent is mainly composed of Bacillus pumilus WP8, Pseudomonas putida sp. RBP1, and Acinetobacter Sp. A6. The method of the invention not only effectively solves the problem of high temperature flatulence existing in the biogas slurry agricultural bio-fertilizer soft packaging, but also effectively improves the fertilizer efficiency of a biogas slurry agricultural fertilizer after treatment, improves the soil abundance and diversity, and soaks the seeds to increase the seed germination rate and effects of disease resistance and other aspects.
Description
technical field [0001] The invention belongs to the technical field of agricultural waste treatment, and in particular relates to a method for eliminating flatulence in flexible packages of biogas slurry agricultural biological fertilizers through the combined use of chemical reagents and composite microbial agents, and the combination of biogas slurry agricultural biological fertilizers, chemical reagents and composite microbial agents Combined methods to improve fertilizer efficiency. Background technique [0002] In recent years, the state has vigorously supported and promoted the harmless treatment and energy utilization of organic waste such as farming manure. This measure can not only eliminate environmental pollution, produce clean energy biogas, and produce biogas slurry and biogas residues to produce organic fertilizers, meet the development needs of energy conservation, emission reduction and renewable energy utilization, and meet the circular economy of farming-pl...
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Login to View More IPC IPC(8): C05G3/00C05G3/04C05G3/80
Inventor 沈敏康贻军夏丹朱德伟印志峰张言周仇婷婷蔡海婕
Owner YANCHENG TEACHERS UNIV