A kind of locust uridine diphosphate glucuronosyltransferase gene and its application

A uridine diphosphate glucose, transferase technology, applied in transferase, application, genetic engineering and other directions, can solve problems such as unfavorable agricultural environment, green, sustainable development, etc., and achieve the effect of reducing selectivity and fitness

Active Publication Date: 2022-05-31
LINYI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, traditional chemical control methods are mostly used for the prevention and control of Asian locusts, which is not conducive to the green and sustainable development of the agricultural environment

Method used

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  • A kind of locust uridine diphosphate glucuronosyltransferase gene and its application
  • A kind of locust uridine diphosphate glucuronosyltransferase gene and its application
  • A kind of locust uridine diphosphate glucuronosyltransferase gene and its application

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Experimental program
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Effect test

Embodiment 1

[0025] 2) The homogenate was transferred to a 1.5mL centrifuge tube, left standing at room temperature for 5min, and centrifuged at 13000r for 5min at 4°C.

[0026] 3) Transfer the supernatant to a clean 1.5mL centrifuge tube, add 200 μL of chloroform, and vortex for 15s. room temperature

[0029] 6) 4 ℃, 13000r centrifugal 2min, discard the filtrate.

[0034] Using PrimeScript

[0036] 2) After being incubated at 65°C for 5 min, rapidly cooled on ice.

[0038] 4) Mix slowly.

[0040] 6) Incubate at 95°C for 5min to inactivate the enzyme, place on ice, and store the cDNA at -20°C.

[0049] PCR reaction conditions are as follows: 95°C for 3 min; 95°C for 30s, 55°C for 30s, 72°C for 1.5min, 35 cycles; 72°C

[0059] The sequencing results show that: PCR amplification obtains a DNA fragment with a size of 1561 bp.

Embodiment 2

[0064] Primers were designed according to the cloned gene fragments, and a T7 promoter was introduced at the 5' end of the primers. The primer sequences are as follows:

[0070] PCR reaction conditions are as follows: 95°C for 3min; 95°C for 30s, 55°C for 30s, 72°C for 1min, 35 cycles; 72°C for 10min;

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Abstract

The invention discloses a locust uridine diphosphate glucuronosyltransferase gene, the nucleotide sequence of which is shown in SEQ ID NO:1. According to the gene, primers are designed to synthesize dsRNA for interfering with uridine diphosphate glucuronosyltransferase gene, and dsRNA is introduced into locusts to perform RNA interference on uridine diphosphate glucuronosyltransferase gene, which can significantly reduce the food selectivity of locusts and fitness, thereby providing a new method for green and sustainable pest control, and also providing technical support for the creation of new biopesticides.

Description

A kind of locust uridine diphosphate glucuronyltransferase gene and its application technical field The present invention relates to the field of biotechnology, more specifically to a kind of locust uridine diphosphate glucuronic acid conversion. Transferase genes and their applications. Background technique [0002] Crops suffering from insect pests can reduce productivity and endanger food security. Traditional pest control methods are mostly chemical control However, it is too dependent on the use of chemical pesticides, which is easy to cause problems such as pest resistance, rampant, and serious residual poisoning. Heavy damage to the ecological environment and the quality and safety of agricultural products. Therefore, develop and create green, efficient and sustainable pest control technologies appear particularly important. The Asian locust Oedaleus asiaticus Bey-Bienko is the most serious hazard in northern my country. One of the dominant species of locu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/54C12N9/10C12N15/89A01K67/033A01N37/46A01P7/04
CPCY02A50/30
Inventor 黄训兵王月月刘文
Owner LINYI UNIVERSITY
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