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Plasma exosome miRNA molecular marker and application thereof in preparation kit

A technology of molecular markers and exosomes, applied in the field of medicine, achieves high clinical use and promotion value, high sensitivity, and good diagnostic effect

Inactive Publication Date: 2019-06-21
ATTACHED OBSTETRICS & GYNECOLOGY OSPITAL MEDICALCOLLEGE ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, circulating miRNAs as diagnostic markers have certain limitations because they are easily affected by miRNAs released from damaged cells.

Method used

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  • Plasma exosome miRNA molecular marker and application thereof in preparation kit
  • Plasma exosome miRNA molecular marker and application thereof in preparation kit
  • Plasma exosome miRNA molecular marker and application thereof in preparation kit

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Embodiment Construction

[0017] The present invention will be further elaborated below through specific examples. It should be noted that the following descriptions are only for explaining the present invention, and do not limit its content.

[0018] The method for obtaining the molecular marker of the present invention is as follows:

[0019] (1) Collect 121 peripheral blood samples including 23 healthy volunteers, 5 patients with precancerous lesion I (CINI), 59 patients with precancerous lesion II to III (CINII-III), and 34 patients with cervical cancer ( CC, including 21 cervical squamous cell carcinoma (SCC) patients and 13 cervical adenocarcinoma (ACC) patients).

[0020] (2) Peripheral blood collected in blood collection tubes containing ACD anticoagulant was centrifuged at 1900×g at 4°C for 10 minutes within 8 hours of blood collection, then carefully sucked out the light yellow supernatant, and then centrifuged at 16,000×g at 4°C After 10 minutes, the upper layer of plasma was divided into 1...

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Abstract

The invention discloses a plasma exosome miRNA molecular marker and an application thereof in a micro-digital PVR (polymerase chain reaction) kit for early diagnosis of cervical cancer. The RNA sequence of the molecular marker is shown in SEQ ID NO: 1-SEQU ID NO:8. Based on the marker, the detection has high sensitivity and specificity, and the AUC can be up to 0.992, and the diagnosis result is good. In the test data, samples of patients with intraepithelial neoplasia below grade 1 and above grade 2 can be effectively distinguished according to the expression levels of two miRNAs. The novel plasma exosome miRNA molecular marker has the characteristics of good diagnostic indicators, the diagnosis kit and the detection are used for detecting the 8 miRNAs, the kit is composed of specific amplification primers and general PRC amplification reagents and can be effectively used for screening and diagnosis of cervical cancer, and high clinical application and promotion value is achieved.

Description

technical field [0001] The invention belongs to the technical field of medicine, in particular to a plasma exosome miRNA biomarker and a kit for early screening and diagnosis of cervical cancer and precancerous lesions. Background technique [0002] With increasing morbidity and mortality, cancer has become a major cause of harm to public health worldwide. The incidence of cervical cancer is 98.9 per 100,000 people, and the death toll is 30.5 per 100,000 people. These two indicators rank second and third respectively among female malignant tumors. According to the latest statistical data, cervical cancer is the second leading cause of cancer death among women aged 20-39, with a wide range of influence and deep harm. The Pap smear test or Thin Pad's liquid-based cytology test in cervical cancer screening has effectively improved the diagnosis rate of early cancer patients or patients with precancerous lesions, greatly reduced the incidence of cervical cancer, and increased t...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12Q1/686C12N15/113
Inventor 陆燕刘鹏渊吕卫国郑梦月
Owner ATTACHED OBSTETRICS & GYNECOLOGY OSPITAL MEDICALCOLLEGE ZHEJIANG UNIV
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