Detection method for Guizhishaoyaozhimu decoction

Abstract

The invention provides a detection method for Guizhishaoyaozhimu decoction. According to the method, high performance liquid chromatography is adopted to detect the Guizhishaoyaozhimu decoction. The method includes the following operation steps of: 1) the preparation of reference solutions: gallic acid, mangiferin, paeoniflorin, prim-O-glucosylcimifugin, liquiritin, 4'-O-beta-glucopyranosyl-5-O-methylvisamminol, cinnamic acid and ammonium glycyrrhizinate reference substances are selected, methanol is added to the above reference substances so as to dissolve the reference substances, and obtained substances are adopted as reference solutions; 2) the preparation of a test solution: Guizhishaoyaozhimu decoction freeze-dried powder is taken, and methanol is added to dissolve the Guizhishaoyaozhimu decoction freeze-dried powder, so that the test solution can be obtained; and 3) detection: the reference solutions and the test solution are separately injected into a liquid chromatograph. Themethod of the invention can be used to not only detect the gallic acid, mangiferin, paeoniflorin, prim-O-glucosylcimifugin, liquiritin, 4'-O-beta-glucopyranosyl-5-O-methylvisamminol, cinnamic acid andammonium glycyrrhizinate, but also realize the overall quality control of the Guizhishaoyaozhimu decoction. The detection method can provide a methodological basis for research on the material basisof the Guizhishaoyaozhimu decoction and has a popularization and application value.

Description

technical field [0001] The invention specifically relates to a detection method of Guizhi Shaoyao Zhimu Decoction. Background technique [0002] Guizhi Shaoyao Zhimu Decoction comes from "Synopsis of the Golden Chamber · Stroke, Lijie, Syndrome, Syndrome and Treatment". It has the functions of expelling wind and dehumidification, warming meridians and dispelling cold, nourishing yin and clearing heat. Rheumatoid arthritis (RA) belongs to the category of arthralgia in traditional Chinese medicine. Modern clinical and pharmacological studies have found that Guizhi Shaoyao Zhimu Decoction has definite curative effect in treating RA. The pathogenesis of RA is not yet clear, but studies have shown that the level of free radicals in the serum and joint fluid of RA patients and model animals increases, and free radicals play an important role in the damage of articular cartilage in RA diseases. Guizhi Shaoyao Zhimu Decoction can Effectively scavenge free radicals. [0003] At pr...

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Problems solved by technology

[0003] At present, the research on Guizhi Shaoyao Zhimu Decoction mainly focuses on its pharmacodynamics research, and there are few reports on its material basis research, such as Dong Yunzhuo. HPLC simultaneously determines the content of 7 active ingredients in Guizhi Shaoyao Zhimu Decoction, Only 7 of them can be analyzed, and cinnamic acid, which has obvious active ingredients, cannot be detected, which cannot effectively reflect the quality of Guizhi Shaoyao Zhimu Decoction

The detection methods disclosed at this stage cannot effectively control the quality of Guizhi Shaoyao Zhimu Decoction

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